植物乳杆菌NCU116的模拟人体肠道上皮细胞黏附性能

doi:10.7506/spkx1002-6630-201315052

食品科学

植物乳杆菌NCU116的模拟人体肠道上皮细胞黏附性能

熊涛，黄巧芬，李萍，黄涛

南昌大学生命科学与食品工程学院，食品科学与技术国家重点实验室，江西南昌 330047

出版日期:2013-08-15 发布日期:2013-09-03
通讯作者: 熊涛
基金资助:
国家“863”计划项目(2011AA100904)；教育部留学回国人员创业基金项目(赣教财字[2009]135号)；
江西省教育厅科技落地计划项目(赣财教 [2011]243号)；国家自然科学基金项目(31060224)

Adhesive Properties of Lactobacillus plantarum NCU116 on Simulated Human Intestinal Epithelial Cells

XIONG Tao，HUANG Qiao-fen，LI Ping，HUANG Tao

State Key Laboratory of Food Science and Technology, School of Life Sciences and Food Engineering, Nanchang University,
Nanchang 330047, China

Online:2013-08-15 Published:2013-09-03
Contact: XIONG Tao

摘要/Abstract

摘要：

益生菌对人体肠道上皮细胞表面的黏附性能是益生菌筛选的重要指标之一。采用体外细胞模型(人体结肠癌细胞系HT-29)测定植物乳杆菌NCU116的黏附性能，并研究不同因素对NCU116黏附HT-29的影响。结果表明：植物乳杆菌NCU116的黏附能力较强，为(4.78±0.21)CFU/cell。黏附菌数随着菌液浓度增大而显著增大，当菌液浓度达到1.0×108CFU/mL时趋于饱和；稳定期的菌体黏附效果较好；黏附菌数随着作用时间的延长而增加，到2.0h时逐渐趋于饱和；外部环境的pH值对黏附性能影响很大，偏酸性环境更有利于黏附；Ca2+、Mg2+对黏附作用没有显著影响。植物乳杆菌NCU116对体外细胞模型HT-29的黏附性能较好，在食品行业和保健业中具有良好的发展前景。

关键词: HT-29细胞, 植物乳杆菌NCU116, 黏附性能

Abstract:

The adherence of probiotics on human epithelial cells is an important criterion for the selection of
probiotics. Colon cancer cell line HT-29 was selected to test the adhesive capability of Lactobacillus plantarum
NCU116 in vitro. Furthermore, the impacts of different factors on the adherence were studied. Results indicated that
the adhesion (4.78 ± 0.21) CFU/cell of Lactobacillus plantarum NCU116 was very excellent. The adhesion of NCU116
became better with increasing concentration, but the growth tended to saturation when the concentration was up to 1.0 × 108
CFU/mL. The adhesion of bacteria in stationary phase was the best. Similarly, the adhesion revealed an enhancement with
increasing incubation time and reached saturation at 2.0 h. The environmental pH had a significant impact on the adhesion
of NCU116, and NCU116 had a better adherence in acidic environment. Neither Ca2+ nor Mg2+ had significant effects on
the adherence of Lactobacillus plantarum NCU116 on HT-29 cells. In summary, Lactobacillus plantarum NCU116 has an
excellent adherence and is promising in the food and healthcare industries.

Key words: HT-29 cells, Lactobacillus plantarum NCU116, adherence

中图分类号:

TS201.3

熊涛，黄巧芬，李萍，黄涛. 植物乳杆菌NCU116的模拟人体肠道上皮细胞黏附性能[J]. 食品科学, doi: 10.7506/spkx1002-6630-201315052.

XIONG Tao，HUANG Qiao-fen，LI Ping，HUANG Tao. Adhesive Properties of Lactobacillus plantarum NCU116 on Simulated Human Intestinal Epithelial Cells[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201315052.

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植物乳杆菌NCU116的模拟人体肠道上皮细胞黏附性能

Adhesive Properties of Lactobacillus plantarum NCU116 on Simulated Human Intestinal Epithelial Cells

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