食品科学

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响应面法优化黄河鲤鱼鱼鳞酸溶性胶原蛋白提取工艺

朱文学,邱园园,肖 枫,康帅飞,王玲玲   

  1. 河南科技大学食品与生物工程学院,河南 洛阳 471023
  • 出版日期:2013-10-25 发布日期:2013-09-28

Optimization of Extraction Process for Acid-Soluble Collagen from Cyprinus carpio Haematopterus Scale by Response Surface Methodology

ZHU Wen-xue,QIU Yuan-yuan,XIAO Feng,KANG Shuai-fei,WANG Ling-ling   

  1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China
  • Online:2013-10-25 Published:2013-09-28

摘要:

以新鲜的黄河鲤鱼鱼鳞为原料,在单因素试验的基础上,采用响应面法Box-Behnken试验设计,对影响酸溶性胶原蛋白提取效率的3个影响因素即乙酸浓度、液料比和提取时间进行优化,建立并分析各因素与酸溶性胶原蛋白得率关系的数学模型。结果表明:黄河鲤鱼鱼鳞酸溶性胶原蛋白的最佳提取工艺条件为提取剂乙酸浓度0.54mol/L、液料比10:1(mL/g)、提取时间42h,在此条件下胶原蛋白的提取率达到15.89%。

关键词: 黄河鲤鱼鱼鳞, 胶原蛋白, 响应面法, 提取工艺, 优化

Abstract:

On the basis of one-factor-at-a-time experiments, Box-Behnken design (BBD) and response surface methodology
(RSM) were applied to explore the effects of acetic acid concentration, material/liquid ratio and extraction duration on the
extraction efficiency of collagen from common carp fish scale. As a result, a mathematical model was fitted. The optimal extraction
conditions were found to be 0.54 mol/L acetic acid as the extraction solvent with a material/liquid ratio of 1:10 (g/mL) for an
extraction duration of 42 h. Under these conditions, the extraction efficiency of collagen was 15.89%.

Key words: Yellow River common carp scale, collagen, response surface methodology, extraction process, optimization