响应面法优化大麦苗叶绿素提取工艺

doi:10.7506/spkx1002-6630-201402014

食品科学 ›› 2014, Vol. 35 ›› Issue (2): 75-80.doi: 10.7506/spkx1002-6630-201402014

响应面法优化大麦苗叶绿素提取工艺

张辉1,2，张娜娜3，马丽1，唐坚3，乔勇进2,*

1.上海理工大学医疗器械与食品学院，上海 200093；2.上海市农业科学院农产品保鲜加工研究中心，上海 201403；
3.上海师范大学生命与环境科学学院，上海 200234

收稿日期:2013-05-09 修回日期:2013-12-23 出版日期:2014-01-25 发布日期:2014-02-19
通讯作者: 乔勇进 E-mail:nd_zh@sina.com
基金资助:
上海市科委国际合作计划项目（073907003）

Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology

ZHANG Hui1,2, ZHANG Na-na3, MA Li1, TANG Jian3, QIAO Yong-jin2,*

1. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China;
2. Agricultural Products Storage and Processing Research Center, Shanghai Academy of Agricultural Science, Shanghai 201403,
China; 3. College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China

Received:2013-05-09 Revised:2013-12-23 Online:2014-01-25 Published:2014-02-19
Contact: QIAO Yong-jin E-mail:nd_zh@sina.com

摘要/Abstract

摘要：

为优化大麦苗叶绿素的提取工艺，试验以乙醇-水为溶剂对大麦苗叶绿素进行浸渍提取。采用Plackett-Burman 试验设计筛选影响大麦苗叶绿素提取量的显著因素，通过中心组合试验设计优化其提取工艺参数。结果表明：液料比、乙醇体积分数及提取时间是影响叶绿素提取量的显著因素。优化所得最佳提取工艺参数为液料比31∶1、乙醇体积分数92%、提取时间112 min。在此条件下，叶绿素提取量为13.622 mg/g，与预测值13.646 mg/g较一致。故响应面法可用于优化大麦苗叶绿素的提取工艺，且其优化工艺参数可为大麦苗叶绿素工业化生产提供参考。

关键词: Plackett-Burman试验设计, 中心组合试验设计, 大麦苗叶绿素, 提取工艺优化

Abstract:

The extraction of chlorophyll from young barley grasses with aqueous ethanol as extraction solvent was
optimized. Significant variables that influence extraction efficiency were selected by Plackett-Burman experimental design
and optimized by central composite experimental design. Solid-to-solvent ratio, ethanol concentration and extraction time
were identified as main influencing parameters. The optimal extraction conditions were found to be 31:1, 92% and 112 min.
Under these conditions, the observed yield of chlorophyll was 13.646 mg/g, approximating to the predicted value of
13.622 mg/g. It can be concluded that response surface methodology is applicable to optimize the parameters for extraction
of chlorophyll from young barley grasses and the optimized extraction process parameters can provide a reference for
industrial production of chlorophyll from young barley grasses.

Key words: Plackett-Burman design, central composite design, chlorophyll of young barley grasses, optimization of extraction process

中图分类号:

R284.2

张辉1,2，张娜娜3，马丽1，唐坚3，乔勇进2,*. 响应面法优化大麦苗叶绿素提取工艺[J]. 食品科学, 2014, 35(2): 75-80.

ZHANG Hui1,2, ZHANG Na-na3, MA Li1, TANG Jian3, QIAO Yong-jin2,*. Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology[J]. FOOD SCIENCE, 2014, 35(2): 75-80.

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响应面法优化大麦苗叶绿素提取工艺

Optimization of Extraction Process for Chlorophyll from Young Barley Grasses Based on Response Surface Methodology

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