采后植酸处理对紫背天葵贮藏期间生理生化反应的影响

doi:10.7506/spkx1002-6630-201402062

食品科学 ›› 2014, Vol. 35 ›› Issue (2): 316-321.doi: 10.7506/spkx1002-6630-201402062

采后植酸处理对紫背天葵贮藏期间生理生化反应的影响

姜丽，蒋娟，张丽，傅淋然，郁志芳*

南京农业大学食品科技学院，江苏南京 210095

收稿日期:2013-02-22 修回日期:2013-12-30 出版日期:2014-01-25 发布日期:2014-02-19
通讯作者: 郁志芳 E-mail:ytjiangli@163.com
基金资助:
南京农业大学青年发展基金项目（KJ2011015）；江苏高校优势学科建设工程资助项目（PAPD）；
国家自然科学基金青年科学基金项目（31301576）；中央高校基本科研业务费专项（KYZ201319）

Physiological and Biochemical Responses of Gynura bicolor D.C to Phytic Acid during Storage

JIANG Li, JIANG Juan, ZHANG Li, FU Lin-ran, YU Zhi-fang*

College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2013-02-22 Revised:2013-12-30 Online:2014-01-25 Published:2014-02-19
Contact: YU Zhi-fang E-mail:ytjiangli@163.com

摘要/Abstract

摘要：

以紫背天葵为试材，采用不同浓度（0.1、0.5 mmol/L和1 mmol/L）植酸处理后，高密度聚乙烯袋（15 μm 厚）挽口包装，并置于0 ℃条件下贮藏15 d，每5 d测定一次生理指标，包括腐烂率、呼吸强度、细胞膜渗透率、多酚氧化酶（PPO）、过氧化物酶（POD）、过氧化氢酶（CAT）、超氧化物岐化酶（SOD）的酶活力和丙二醛（MDA）和超氧阴离子自由基（O2 － ·）含量。结果表明，所有植酸处理组均可不同程度的抑制采后紫背天葵的生理生化反应，并延缓衰老。其中，0.5 mmol/L植酸处理有效减小MDA和O2 － ·的积累，维持了较好的细胞膜的完整性，在抑制呼吸速率和PPO活性、增强POD和SOD活性方面也效果显著，由此说明，0.5 mmol/L植酸处理可以有效提高紫背天葵贮藏生理品质，延缓衰老。

关键词: 紫背天葵, 植酸, 保鲜

Abstract:

The effect of pretreatment with phytic acid (PA) on physiological and biochemical changes of Gynura bicolor
D.C during storage was investigated. Gynura bicolor D.C leaves were treated with 0.1, 0.5 and 1 mmol/L PA, respectively
and control samples were those treated with water. Both experimental and control samples were wrapped in 15 μm thick
high-density polypropylene (HDPE) bags and stored at 0 ℃ for 15 days. Physiological and biochemical indices, including
decay index, respiration rate, relative leakage, the activities of polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT)
and superoxide dismutase (SOD), and the contents of malondialdehyde (MDA) and superoxide anion free radicals (O2 － ·),
were tested every 5 days. All PA treatments inhibited obvious physiological and biological reactions in Gynura bicolor D.C
leaves. Particularly, 0.5 mmol/L PA delayed the senescence of Gynura bicolor D.C leaves effectively by decreasing the
incidence of decay, maintaining low relative leakage, retarding the accumulation of O2 － · and MDA, inhibiting respiration
rate and PPO activity, and enhancing the activities of POD and SOD. Therefore, PA treatment is potentially applicable in
extending the storage life of Gynura bicolor D.C.

Key words: Gynura bicolor D.C, phytic acid, storage

中图分类号:

TS255.3

姜丽，蒋娟，张丽，傅淋然，郁志芳*. 采后植酸处理对紫背天葵贮藏期间生理生化反应的影响[J]. 食品科学, 2014, 35(2): 316-321.

JIANG Li, JIANG Juan, ZHANG Li, FU Lin-ran, YU Zhi-fang*. Physiological and Biochemical Responses of Gynura bicolor D.C to Phytic Acid during Storage[J]. FOOD SCIENCE, 2014, 35(2): 316-321.

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采后植酸处理对紫背天葵贮藏期间生理生化反应的影响

Physiological and Biochemical Responses of Gynura bicolor D.C to Phytic Acid during Storage

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