关键词: 高粱原花青素, 黏附, 荧光标记, 双向凝胶电泳, 基质辅助激光解吸电离飞行时间质谱

Abstract:

In this study, high purity proanthocyanidins (SPC) and proanthocyanidins tetramers (SPC-III) from sorghum
episperm were compared for their inhibitory effects on the adhesion of Streptococcus mutans Ingbritt (c) through
fluorescence labeling. By combining two-dimensional electrophoresis (2-DE) and matrix-associated laser dissociation/
ionization time of flight mass spectrometry (MALDI-TOF-MS), the major intervening pathways were investigated, and the
underlying mechanisms were explored. The results showed that SPC-III (tetramers) had better inhibitory effect than SPC and
had stronger inhibitory effect than SPC-III-treated salivary acquired pellicle on the adhesion of S. mutans Ingbritt (c), which
was the major pathway to inhibit bacterial adhesion. Two-dimensional electrophoretograms of 3.10-II treated by SPC-III or
not showed significant differences. All different points were different proteases derived from S. mutans, in which A24 was
suggested to be a new protein that has never been detected in S. mutans previously.

Key words: sorghum proanthocyanidins, adhesion, fluorescence labeling, two-dimensional electrophoresis, matrixassociated laser dissociation/ionization time of flight mass spectrometry