鸡枞菌ITS区克隆、测序及其系统发育关系

doi:10.7506/spkx1002-6630-201417036

食品科学

鸡枞菌ITS区克隆、测序及其系统发育关系

龙梅，郭放，郭莉娟，何雪梅，刘小艳，李蓓，罗燕，邹立扣

1.四川农业大学资源与环境学院，四川成都 611130；
2.四川农业大学都江堰校区微生物学实验室，森林资源保护与利用实验室，四川都江堰 611830

出版日期:2014-09-15 发布日期:2014-09-12

Cloning, Sequencing and Phylogenetic Relationships of ITS Region of rDNA from Termitomyces

LONG Mei, GUO Fang, GUO Li-juan, HE Xue-mei, LIU Xiao-yan, LI Bei, LUO Yan, ZOU Li-kou

1. College of Resources and Environment, Sichuan Agricultural University, Chengdu 611130, China; 2. Laboratory of Microbiology,
Laboratory of Forestry Resource Conservation and Utilization, Dujiangyan Campus of Sichuan Agricultural University,
Dujiangyan 611830, China

Online:2014-09-15 Published:2014-09-12

摘要/Abstract

摘要：

采集鸡枞菌（Termitomyces）子实体，对其进行rDNA-ITS 区序列聚合酶链式反应扩增测序，利用MEGA5对rDNA-ITS不同区域作序列分析，并构建鸡枞菌转录间隔区（internal transcribed spacer，ITS）系统发育树。结果表明：在10 种鸡枞菌中，测序结果表明鸡枞菌rDNA-ITS区长度在527～661 bp。系统发育树表明，10 种鸡枞菌中，有8 种鸡枞菌各为一支，尖盾鸡枞菌与球盖白蚁伞聚为一支；待定种A1、E1、H1独为一支，可能为新种；B1、G1、D1可能为谷堆鸡枞菌；I1可能为根白蚁伞；ZZ1可能为粗柄鸡枞菌；待定种C1不能明确。结果证明，ITS1及ITS1-5.8S rDNA-ITS2可用于鸡枞菌进行种间系统发育树的建立，但ITS1区构建的鸡枞菌种间系统发育树支持率最高，ITS2区可辅助进行某些待定种的鉴定。

关键词: 鸡枞菌, 系统发育, ITS序列

Abstract:

The fruit bodies of Termitomyces were collected, and total DNA was extracted as the template to amplify internal
transcribed spacer (ITS). Subsequently, the PCR products were sequenced, and the phylogenetic tree of Termitomyces was
established based on different parts of ITS region by the MEGA5 software. The results showed that the rDNA-ITS region
of 10 different species of Termitomyces was between 527 bp and 661 bp in length. The phylogenetic tree showed that there
were eight branches of Termitomyces. T. clypeatus and T. globules were gathered together. The unknown species A1, E1 and
H1 which were in a single branch may be new species. B1, G1, and D1 could be identified as T. heimii. In addition, I1 could
be T. eurrhizus, and ZZ1 could be T. robustus. However, C1 could not be determined based on the present study. The results
showed that the region of ITS1 and ITS1-5.8S rDNA-ITS2 could be used to build the phylogenetic tree for interspecific
Termitomyces. The phylogenetic tree of ITS1 had a significantly higher supporting rate, and the ITS2 could be helpful
for the confirmation of some unknown species. Therefore, this work has laid a foundation for the classification system of
Termitomyces and provided ITS region sequence information for the identification at the molecular level.

Key words: Termitomyces, phylogenetic tree, ITS region

中图分类号:

TS255.1

龙梅，郭放，郭莉娟，何雪梅，刘小艳，李蓓，罗燕，邹立扣. 鸡枞菌ITS区克隆、测序及其系统发育关系[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417036.

LONG Mei, GUO Fang, GUO Li-juan, HE Xue-mei, LIU Xiao-yan, LI Bei, LUO Yan, ZOU Li-kou. Cloning, Sequencing and Phylogenetic Relationships of ITS Region of rDNA from Termitomyces[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417036.

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鸡枞菌ITS区克隆、测序及其系统发育关系

Cloning, Sequencing and Phylogenetic Relationships of ITS Region of rDNA from Termitomyces

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