根皮苷通过下调小肠NPC1L1和HMG-CoA还原酶表达降低血液胆固醇水平

doi:10.7506/spkx1002-6630-201417037

食品科学

根皮苷通过下调小肠NPC1L1和HMG-CoA还原酶表达降低血液胆固醇水平

申婷婷，刘素稳，赵江，汪名春，常志勇，王浩

1.天津科技大学生物工程学院，天津 300457；2.河北科技师范学院食品科技学院，河北秦皇岛 066604；
3.天津科技大学食品工程与生物技术学院，天津 300457；4.安徽农业大学食品科学与工程系，安徽合肥 230036

出版日期:2014-09-15 发布日期:2014-09-12

Phlorizin Decreases Serum Cholesterol by Downregulating Intestinal NPC1L1 and HMG-CoA Reductase

SHEN Ting-ting, LIU Su-wen, ZHAO Jiang, WANG Ming-chun, CHANG Zhi-yong, WANG Hao

1. College of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
2. College of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066604, China;
3. College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China;
4. Department of Food Science and Engineering, Anhui Agricultural University, Hefei 230036, China

Online:2014-09-15 Published:2014-09-12

摘要/Abstract

摘要：

以高脂高胆固醇膳食喂饲仓鼠为动物模型，研究根皮苷对仓鼠血脂水平及小肠胆固醇代谢相关基因的调控影响。36 只实验动物随机分成对照组和3 个不同剂量根皮苷干预组（3、6、9 g/kg），测定血清中总胆固醇（total cholesterol，TC）、甘油三酯（triglyceride，TG）及高密度脂蛋白胆固醇（high density lipoproteincholesterol，HDL-C）水平，气相色谱法检测肝脏中胆固醇含量及粪便固醇排泄量，实时定量荧光聚合酶链式反应（real-time polymerase chain reaction，Real-Time PCR）分析小肠胆固醇合成、吸收、转化及排泄基因的表达水平。血脂测定结果显示，血清TC、TG水平随给予根皮苷添加量增加而降低，且高剂量组具有显著性（P＜0.05或P＜0.01）；6、9 g/kg根皮苷剂量组，血清中HDL-C水平极显著升高（P＜0.01）。气相色谱检测结果显示，根皮苷剂量组仓鼠肝脏中胆固醇含量较对照组随根皮苷剂量增加而降低，且6、9 g/kg组具有极显著差异（P＜0.01）；粪便中总中性固醇排泄量与根皮苷给予量正相关，且都具有显著性差异（P＜0.05或P＜0.01），总酸性固醇排泄量根皮苷剂量组相比对照组增加，且6 g/kg组显著增加（P＜0.05），9 g/kg组极显著增加（P＜0.01）。Real-Time PCR检测结果显示，根皮苷剂量组小肠中胆固醇合成限速酶HMG-CoA还原酶、小肠胆固醇吸收关键蛋白NPC1L1、胆固醇酯化酶ACAT2、微粒体转运蛋白MTP的mRNA表达水平较对照组显著降低（P＜0.05或P＜0.01）；给予根皮苷后，肠道中促进胆固醇向外排泄基因ABCG5/8表达水平显著升高（P＜0.05或P＜0.01）。因此，根皮苷对高脂高胆固醇膳食饲喂仓鼠胆固醇代谢平衡的调节可能是通过对小肠胆固醇吸收、转化等基因表达的抑制，上调胆固醇排泄基因的表达实现的。

关键词: 根皮苷, 仓鼠, 小肠, 血脂, 基因表达

Abstract:

Destroying the balance of plasma cholesterol into hypercholesterolemia is a major risk factor for atherosclerosis.
The aim of this report was to investigate the effects of phlorizin on blood cholesterol level and gene expression of cholesterolregulating
enzymes in Golden Syrian hamsters maintained on a 0.1% cholesterol high fat diet. Totally 36 hamsters were
randomly divided into control group and three experimental groups with 3, 6, and 9 g/kg phlorizin, and serum total
cholesterol (TC), triacylglycerols (TG) and high-density-lipoprotein-cholesterol (HDL-C) were detected. Then, the contents
of cholesterol in liver and fecal neutral and acidic sterols were determined by GC. The gene expression of cholesterolregulating
proteins in the small intestine was assayed with Real-Time PCR. Serum TC and TG were significantly decreased
in 9 g/kg phlorizin group compared with those in the control group, while HDL-C in 6 and 9 g/kg phlorizin groups were
significantly increased (P ＜ 0.01). The hepatic cholesterol level in the experimental groups supplemented with 6 and 9 g/kg
phlorizin was significantly lower than that in the control group (P ＜ 0.01). Higher excretion of fecal cholesterol was observed
in the phlorizin groups. The amount of total fecal neutral sterols was increased compared with that in the control group
(P ＜ 0.05 or P ＜ 0.01). The excretion of total fecal acidic sterols was increased as the amount of phlorizin increased (6 g/kg,
P ＜ 0.05; 9 g/kg, P ＜ 0.01). It was also found that the cholesterol-lowering activity of phlorizin was associated with downregulation
of intestinal 3-hydroxy-3-methyl glutaryl-CoA (HMG-CoA) reductase, niemann-pick C1-like 1 (NPC1L1), acyl-
CoA-cholesterol acyltransferase 2 (ACAT2), microsomal triacylglycerol transport protein (MTP), and up-regulation of ATPbinding
cassette transporter such as subfamily G member 5 and 8 (ABCG5/8) transporters. The mechanisms underlying the
cholesterol-lowering activity of phlorizin were mediated most likely by increasing the sterol excretion and decreasing the
cholesterol absorption and synthesis.

Key words: phlorizin, hamsters, small intestine, serum lipid, gene expression

中图分类号:

R151.2

申婷婷，刘素稳，赵江，汪名春，常志勇，王浩. 根皮苷通过下调小肠NPC1L1和HMG-CoA还原酶表达降低血液胆固醇水平[J]. 食品科学, doi: 10.7506/spkx1002-6630-201417037.

SHEN Ting-ting, LIU Su-wen, ZHAO Jiang, WANG Ming-chun, CHANG Zhi-yong, WANG Hao. Phlorizin Decreases Serum Cholesterol by Downregulating Intestinal NPC1L1 and HMG-CoA Reductase[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201417037.

[1]	詹慧，宋田源，余刚，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 基于酶活力模型和细胞模型分析红毛藻多糖降血脂活性[J]. 食品科学, 2021, 42(7): 142-148.
[2]	范明，彭丽桃，闫等，范刚，杨书珍，李杰. VmaH和PMA在指状青霉中的表达及其作为潜在杀菌作用靶点的可能性[J]. 食品科学, 2021, 42(6): 126-133.
[3]	范三红，王娇娇，李佳妮，白宝清. 辣椒红色素和辣椒碱体内抗氧化活性及降血脂功能[J]. 食品科学, 2021, 42(5): 201-207.
[4]	赵乐，张晓桐，刘利军，谢水琪，靳奇文，孟祥晨. 氨基酸对植物乳杆菌KLDS1.0391生长及细菌素合成的影响[J]. 食品科学, 2021, 42(18): 37-44.
[5]	贺明阳，洪敏，王日葵，喻最新，朱莉，周炼，邓涂静. 宽皮柑橘浮皮时脱落酸积累变化及其代谢基因表达特征[J]. 食品科学, 2021, 42(17): 54-62.
[6]	戚英伟，刘悬烁，丁毓端，姜永华，张玉洁，刘佳，蒋紫洮，任小林. 脱落酸处理对苹果成熟、乙烯合成及其信号转导基因表达的影响[J]. 食品科学, 2021, 42(17): 225-232.
[7]	王瑾，白羽嘉，冯作山，朱婉彤，曾禹睿慷，蒋莉莹. 链格孢侵染采后甜瓜病程相关蛋白基因表达及酶活性变化规律[J]. 食品科学, 2021, 42(14): 94-102.
[8]	魏连会，宋淑敏，董艳，石杰，王娇娇，李宝龙，孙兴荣. 火麻籽多肽对高脂饮食喂养大鼠血脂的影响[J]. 食品科学, 2021, 42(11): 161-167.
[9]	高渊，杨亚茹，常明昌，孟俊龙，刘靖宇，冯翠萍. 基于代谢组学研究绣球菌多糖对高脂血症大鼠的降血脂作用[J]. 食品科学, 2021, 42(11): 168-175.
[10]	李志春，陈赶林，郑凤锦，孙健，林波，方晓纯. 甘蔗醋对高脂小鼠血脂代谢及组织氧化还原的影响[J]. 食品科学, 2020, 41(9): 86-90.
[11]	温永平，朱迪，孙健，闫巧娟，江正强. 魔芋甘露寡糖抗肥胖活性及机制[J]. 食品科学, 2020, 41(5): 115-121.
[12]	刘兴龙，赵迎春，陈雪艳，佘欣鑫，SADIA Khatoon，姜颖，解慧勇，蒋白楠，郑毅男，刘文丛，丁传波. 黑参多糖抗疲劳作用的分子机制[J]. 食品科学, 2020, 41(5): 173-179.
[13]	冯帅帅，闫成太，张天皓，张莉，吉苗苗，王帆，高华. 套袋对‘瑞雪’苹果香气成分的影响及相关基因表达分析[J]. 食品科学, 2020, 41(4): 185-192.
[14]	张舒翼，丑述睿，崔惠军，王撼辰，刘璇，李斌. 酸性条件下根皮苷对低酯果胶流变特性、抗氧化活性及微观结构的影响[J]. 食品科学, 2020, 41(2): 43-49.
[15]	崔敬爱，王思霁，刘畅，于婷，陈晓平. 桦褐孔菌多糖对高脂饮食诱导的高脂血症大鼠血脂和肝脏的保护作用及机制[J]. 食品科学, 2020, 41(19): 185-190.

根皮苷通过下调小肠NPC1L1和HMG-CoA还原酶表达降低血液胆固醇水平

Phlorizin Decreases Serum Cholesterol by Downregulating Intestinal NPC1L1 and HMG-CoA Reductase

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价