食品科学

• 工艺技术 • 上一篇    下一篇

苜蓿冰结构蛋白的提取及对冷冻面团的影响

曲 敏,董正婷,陈凤莲,刘羽佳,李凌俐,孙兆国,鲍 欢   

  1. 哈尔滨商业大学 食品科学与工程省级重点实验室,黑龙江 哈尔滨 150076
  • 出版日期:2014-12-25 发布日期:2014-12-29
  • 基金资助:

    黑龙江省自然科学基金项目(C2011-24);黑龙江省教育厅科学技术研究项目(12511128);
    黑龙江省博士后科研启动基金项目(LBH-Q13098)

Extraction of Alfalfa Ice Structuring Proteins and Their Effect on Frozen Dough

QU Min, DONG Zheng-ting, CHEN Feng-lian, LIU Yu-jia, LI Ling-li, SUN Zhao-guo, BAO Huan   

  1. Key Laboratory of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2014-12-25 Published:2014-12-29

摘要:

以“肇东”紫花苜蓿干草为原料。利用磷酸盐缓冲溶液法提取苜蓿蛋白,通过单因素和响应曲面试验,得到提取苜蓿蛋白的最佳工艺条件为料液比1∶20、磷酸盐缓冲溶液浓度63 mmol/L、pH 7.7、草粉粉碎粒度80 目、搅拌时间2 h。在此条件下苜蓿蛋白的提取率为37.77%。经硫酸铵沉淀,透析脱盐,冷冻干燥后复溶,对其溶液进行冰晶形态观察,可以看出苜蓿蛋白的冰晶体积小、数量多、形态多样,说明其抑制冰晶生长及对冰晶的形态修饰效果显著,具有很好的抗冻活性,为冰结构蛋白。将其应用于冷冻面团后,冷冻面团的硬度明显降低,弹性、回复性和内聚性稍增强,咀嚼性有所降低,质地细腻柔软。

关键词: 苜蓿, 冰结构蛋白, 冰晶, 冷冻面团

Abstract:

In the present study, proteins were extracted from the young stems with leaves of Zhaodong alfalfa with
phosphate buffer solution. Furthermore, the optimal extraction conditions for alfalfa proteins were determined by singlefactor
experiment and response surface methodology. The results revealed that when the extraction was carried out by 2 h
stirring of the 80-mesh raw material powder suspended in 63 mmol/L phosphate buffer solution at pH 7.7 with a solid/liquid
ratio of 1:20, the yield of alfalfa proteins was 37.77%. The observed ice crystal morphology suggested that the ice crystals
of the purified alfalfa proteins were small in size, increased in number and varied morphologically, indicating that the alfalfa
proteins are able to significantly inhibit the growth and modify the conformations of ice crystals. These results demonstrate
that the alfalfa proteins, which possess excellent antifreeze activity, are ice structuring proteins. In addition, we characterized
the functional role of these alfalfa proteins in practical application by application in frozen dough. The results of texture
properties demonstrated that the frozen dough mix had a soft and delicate texture, and increased elasticity, resilience and
cohesion, but reduced chewiness.

Key words: alfalfa, ice structuring protein, ice crystal, frozen dough

中图分类号: