食品科学

• 生物工程 • 上一篇    下一篇

嗜冷普鲁兰酶产生菌NX-1的筛选及产酶条件优化

康怀彬1,肖天天1,李净净1,杨 桥2,杨华田1,尤晓颜1,*   

  1. 1.河南科技大学食品与生物工程学院,河南 洛阳 471023;2.中国水产科学研究院东海水产研究所,上海 200090
  • 出版日期:2015-01-15 发布日期:2015-01-16

Screening of Psychrophilic Strain NX-1, a Pullulanase-Producing Bacterium and Optimization of Culture Conditions for Pullulanase Production

KANG Huaibin1, XIAO Tiantian1, LI Jingjing1, YANG Qiao2, YANG Huatian1, YOU Xiaoyan1,*   

  1. 1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China;
    2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
  • Online:2015-01-15 Published:2015-01-16

摘要:

从南极海泥样品中分离得到一株产嗜冷普鲁兰酶的假交替单胞菌菌株(Pseudoalteromonas sp.)NX-1。以菌株NX-1为研究对象,对菌株NX-1产嗜冷普鲁兰酶的酶学性质进行了初步研究,并通过单因素试验和正交试验对其产嗜冷普鲁兰酶的最佳培养条件进行研究。结果显示,菌株NX-1所产嗜冷普鲁兰酶的最适作用温度为20 ℃,最适作用pH值为7.0,在最适条件下,酶的半衰期约为120 min;菌株NX-1的最适产酶条件为:培养温度20 ℃、接种量1%、培养基各组分含量分别为蔗糖15 g/L、蛋白胨15 g/L、CaCl2 0.5 g/L、Na2HPO4 6 g/L。优化后菌株产酶可达25.172 U/mL,相比优化前提高25.1%。

关键词: 嗜冷普鲁兰酶, 假交替单胞菌, 发酵, 正交试验

Abstract:

In the present study, a psychrophilic pullulanase-producing strain, Pseudoalteromonas sp. NX-1, was isolated
from Antarctic marine sediment samples with optimum growth temperature of 20 ℃. The enzymatic properties of
pullulanase from strain NX-1 were investigated. The medium composition and culture conditions for pullulanase production
by NX-1 were optimized by single factor and orthogonal array experiments. The results showed the optimum catalytic pH of
the pullulanase was 7.0, and the half-life was 120 min at optimal catalytic conditions. The pullulanase activity of this isolate
was influenced in decreasing order by the following medium components sugar, peptone, CaCl2 and Na2HPO4. The optimal
culture conditions were 20 ℃ and an inoculum size of 1% using a medium consisting of 15 g/L sugar, 15 g/L peptone,
0.5 g/L CaCl2 and 6 g/L Na2HPO4. The yield of pullulanase was improved by 25.1% when compared to that obtained before
optimization that before.

Key words: psychrophilic pullulanase, Pseudoalteromonas, fermentation, orthogonal experiments

中图分类号: