食品科学

• 工艺技术 • 上一篇    下一篇

棉籽壳酶法制备低聚木糖工艺条件优化

丁长河,张洪宾,周迎春,马 康,殷丽君   

  1. 河南工业大学粮油食品学院,河南 郑州 450001
  • 出版日期:2015-02-25 发布日期:2015-02-16
  • 基金资助:

    河南省科技成果转化基金项目(132201610014);河南工业大学研究生教育创新计划基金项目(2012YJCX22)

Optimization of Extraction Conditions for Xylo-oligosaccharides from Cotton Seed Husk

DING Changhe, ZHANG Hongbin, ZHOU Yingchun, MA Kang, YIN Lijun   

  1. College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China
  • Online:2015-02-25 Published:2015-02-16

摘要:

对棉籽壳酶法生产低聚木糖的工艺参数进行优化。采用响应面法优化了预处理时间、盐酸溶液质量分数和酶解pH值,得到最佳工艺条件为:预处理时间35.70 min、盐酸溶液质量分数0.17%、酶解pH 6.5。在此条件下,木二糖质量浓度达到14.52 g/L,与模型预测值基本相符。且低聚木糖以木二糖为主,木二糖得率为8.13%(以棉籽壳干质量计)。

关键词: 棉籽壳, 低聚木糖, 木二糖, 响应面法

Abstract:

The enzymatic hydrolysis of cotton seed husk with xylanase for xylo-oligosaccharides production was optimized
by response surface methodology in this research. A pretreatment time of 35.70 min with 0.17% HCl was found optimal
for the enzymatic hydrolysis, which was performed at an initial pH of 6.5. At the end of the hydrolysis, the concentration
of xylobiose in hydrolysis was measured to be 14.52 g/L, agreeing with the predicted value. The final product was mostly
xylobiose with a yield of 8.13% (relative to the dry weight of cotton seed husk).

Key words: cottonseed husk, xylo-oligosaccharides, xylobiose, response surface methodology

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