食品科学

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植物乳杆菌在不同盐质量浓度下生长至对数期中期全蛋白SDS-PAGE分析

乌日娜1,2,徐 鑫1,王茜茜1,宋雪飞1,薛雅婷1,唐筱扬1,武俊瑞1,*   

  1. 1.沈阳农业大学食品学院,辽宁 沈阳 110866;2. 江南大学食品学院,食品科学与技术国家重点实验室,江苏 无锡 214122
  • 出版日期:2015-12-15 发布日期:2015-12-24

SDS-PAGE Analysis of Total Proteins in Lactobacillus plantarum in the Middle Logarithmic Growth at Different Salt Concentrations

WU Rina1,2, XU Xin1, WANG Qianqian1, SONG Xuefei1, XUE Yating1, TANG Xiaoyang1, WU Junrui1,*   

  1. 1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 2. State Key Laboratory of Food Science and Technology, College of Food Science, Jiangnan University, Wuxi 214122, China
  • Online:2015-12-15 Published:2015-12-24

摘要:

以耐盐植物乳杆菌FS5-5为研究对象,采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳技术构建了菌株在NaCl质量浓度分别为0、3、6、9 g/100 mL的培养基中生长至对数期中期的全蛋白表达图谱。通过比较分析,选取了6 个差异蛋白质条带,并采用液相色谱-质谱/质谱联用对差异蛋白质条带进行质谱分析。结果表明:1号样品条带鉴定得到6 种蛋白;2号样品条带鉴定得到11 种蛋白;3号样品条带鉴定得到9 种蛋白;4号样品条带鉴定得到4 种蛋白;5号样品条带鉴定得到15 种蛋白;6号样品条带鉴定得到15 种蛋白。去除相同的蛋白,共有45 种蛋白得到鉴定。这些蛋白大致可以分为4 类:与蛋白质合成有关的蛋白25 种;与代谢相关的蛋白10 种;与核苷酸合成有关的蛋白8 种;未知功能蛋白2 种。可能由于这些蛋白的表达发生变化,才导致菌体中蛋白质合成、能量代谢、DNA复制能够正常进行,最终使植物乳杆菌FS5-5能够更好地在盐环境下生存下去。

关键词: 植物乳杆菌FS5-5, 盐胁迫, 十二烷基硫酸钠-聚丙烯酰胺凝胶电泳, 差异蛋白

Abstract:

In this study, the total protein profiles of Lactobacillus plantarum FS5-5 with salt tolerance grown in media
with NaCl concentrations of 0, 3, 6, and 9 g/100 mL to the middle logarithmic phase were established and compared by
sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and six different protein bands were selected for
analysis by liquid chromatography-tandem mass spectrometry (AB Sciex 4000 QTRAP®LC/MS/MS). Results showed that
6, 11, 9, 4, 15 and 15 proteins were contained in bands 1, 2, 3, 4, 5 and 6, respectively. Totally 45 proteins were identified.
These proteins could be divided into 4 groups including 25 proteins involved in protein synthesis, 10 proteins associated
with metabolism, 8 proteins associated with nucleotide synthesis, and 2 proteins with unknown functions. The differential
expression of these proteins could lead to bacterial protein synthesis, energy metabolism and DNA replication, and
eventually enable Lactobacillus plantarum FS5-5 to better survive in salt stress environment.

Key words: Lactobacillus plantarum FS5-5, salt stress, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), different proteins

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