大孔吸附树脂-聚酰胺联用纯化茅岩莓总黄酮工艺优化

doi:10.7506/spkx1002-6630-201612003

食品科学

大孔吸附树脂-聚酰胺联用纯化茅岩莓总黄酮工艺优化

金慧鸣，郭红英，谭兴和*，刘梦浩，王锋，蔡文，张喻，邓洁红

湖南农业大学食品科技学院，食品科学与生物技术湖南省重点实验室，湖南长沙 410128

出版日期:2016-06-25 发布日期:2016-06-29
通讯作者: TAN Xinghe
基金资助:
国家自然科学基金面上项目（31371817）；湖南省自然科学基金项目（2015JJ3076）

Purification of Total Flavonoids from Ampelopsis grossedentata by Combined Use of Macroporous Adsorption Resin and Polyamide

JIN Huiming, GUO Hongying, TAN Xinghe*, LIU Menghao, WANG Feng, CAI Wen, ZHANG Yu, DENG Jiehong

Key Laboratory for Food Science and Bio-Technology of Hunan Province, College of Food Science and Technology,
Hunan Agricultural University, Changsha 410128, China

Online:2016-06-25 Published:2016-06-29

摘要/Abstract

摘要：

为纯化茅岩莓总黄酮，先用HPD-100型大孔吸附树脂（macroporous adsorption resin，MAR）层析柱进行初步纯化，再用聚酰胺（polyamide，PA）层析柱进行第2次纯化，得到的HPD-100型MAR最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速1 mL/min、上样液体积130 mL，在此条件下吸附率为97.14%；最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积40 mL，在此条件下解吸率为94.10%。经HPD-100型MAR纯化后的总黄酮纯度从55.00%提高到了72.25%。PA的最适宜吸附工艺参数为上样液总黄酮质量浓度6 mg/mL、上样流速2 mL/min，在此条件下吸附率为99.57%；最适宜解吸工艺参数为洗脱液乙醇体积分数70%、洗脱流速1 mL/min、洗脱液体积55 mL，在此条件下解吸率为76.50%。经PA纯化后总黄酮纯度从72.25%提高到了80.75%。该方法为茅岩莓黄酮的纯化提供了一种更高效的方法，具有良好的应用前景。

关键词: 茅岩莓, 总黄酮, 大孔吸附树脂, 聚酰胺, 纯化

Abstract:

Sequential column chromatographies on HPD-100 macroporous adsorption resin (MAR) and polyamide (PA)
were used to purify total flavonoids from Ampelopsis grossedentata. The optimum adsorption parameters for HPD-100 MAR
were determined as follows: sample concentration, 6 mg/mL; sample flow rate, 1 mL/min; and sample volume, 130 mL.
Under these conditions, the percentage adsorption was 97.14%. The optimum desorption parameters were established as
follows: eluent concentration, aqueous ethanol (70:30, V/V); eluent volume, 40 mL; and flow rate, 1 mL/min. Under these
conditions, the percentage desorption was 94.10%. After the treatment with HPD-100 MAR, the purity of total flavonoids
was increased from 55.00% to 72.25%. The optimum adsorption parameters for PA were determined as follows: sample
concentration, 6 mg/mL; and flow rate, 2 mL/min. Under these conditions, the percentage adsorption was 99.57%. The
optimum desorption parameters were determined as follows: 70% ethanol in water (V/V) as the eluent; eluent volume,
55 mL; and flow rate, 1 mL/min. Under these conditions, the percentage desorption was 76.50%. After the treatment with
PA, the purity of total flavonoids was further increased to 80.75%. The method presented in this study can provided a
promising approach for the efficiency purification of total flavonoids from Ampelopsis grossedentata.

Key words: Ampelopsis grossedentata, total flavonoids, macroporous adsorption resin, polyamide, purification

中图分类号:

TS201.1

金慧鸣，郭红英，谭兴和，刘梦浩，王锋，蔡文，张喻，邓洁红. 大孔吸附树脂-聚酰胺联用纯化茅岩莓总黄酮工艺优化[J]. 食品科学, doi: 10.7506/spkx1002-6630-201612003.

JIN Huiming, GUO Hongying, TAN Xinghe, LIU Menghao, WANG Feng, CAI Wen, ZHANG Yu, DENG Jiehong. Purification of Total Flavonoids from Ampelopsis grossedentata by Combined Use of Macroporous Adsorption Resin and Polyamide[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201612003.

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大孔吸附树脂-聚酰胺联用纯化茅岩莓总黄酮工艺优化

Purification of Total Flavonoids from Ampelopsis grossedentata by Combined Use of Macroporous Adsorption Resin and Polyamide

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