壳寡糖对Amyloid-β1-42致痴呆大鼠的学习记忆及血清抗氧化功能的影响

doi:10.7506/spkx1002-6630-201701037

食品科学

壳寡糖对Amyloid-β1-42致痴呆大鼠的学习记忆及血清抗氧化功能的影响

李筱筱1，武雪玲1，贾世亮1，张静1，戴雪伶2，孙雅煊1,2,*

1.北京联合大学应用文理学院，北京 100191；2.北京联合大学生物活性物质与功能食品北京市重点实验室，北京 100191

出版日期:2017-01-15 发布日期:2017-01-16

Effect of Chitosan Oligosaccharide on Learning and Memory Functions and Serum Antioxidant Status in a Rat Model of Amyloid-β1–42-Induced Alzheimer’s Disease

LI Xiaoxiao1, WU Xueling1, JIA Shiling1, ZHANG Jing1, DAI Xueling2, SUN Yaxuan1,2,*

1. College of Appllied Arts and Science, Beijing Union University, Beijing 100191, China;
2. Beijing Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191, China

Online:2017-01-15 Published:2017-01-16

摘要/Abstract

摘要： 目的：探讨壳寡糖（chitosan oligosaccharide，COS）对Aβ1-42致痴呆大鼠学习记忆及血清抗氧化功能的影响及其作用机制。方法：采用海马区微注射Aβ1-42建立阿尔茨海默病大鼠痴呆模型，并使用COS干预，通过Morris水迷宫实验观察COS对阿尔茨海默病大鼠学习记忆能力的影响，同时通过测定血清中谷胱甘肽过氧化物酶（glutathioneperoxidase，GSH-Px）和超氧化物歧化酶（superoxide dismutase，SOD）等抗氧化酶的活力以及蛋白质羰基和丙二醛（malondialdehyde，MDA）含量变化观察COS的抗氧化能力。结果：经行为学测试，与假手术对照组相比，模型组大鼠的学习记忆能力明显下降；COS干预后，其学习记忆功能力有所改善。同时，模型组大鼠血清中的SOD和GSH-Px活力相比较假手术组显著降低，MDA和蛋白质羰基含量显著增加；经COS干预后，与模型组相比，大鼠血清中SOD和GSH-Px活力显著上升，MDA和蛋白质羰基含量均显著减少。结论：COS对海马区微注射Aβ1-42致痴呆大鼠有一定的改善和保护作用，具体的作用机制可能与COS的抗氧化作用有关。

关键词: 壳寡糖, &beta, -淀粉样蛋白, 阿尔茨海默病, 氧化应激

Abstract: The objective of the present study was to evaluate the protective effect of chitosan oligosaccharides
(COS) against cognitive deficits and oxidative damage induced by bilateral intrahippocampal (IH) injections of aggregated
amyloid-β1-42 (Aβ1–42) and to explore the underlying molecular mechanisms. Methods: Morris water maze test was performed
to observe the effect of COS on learning and memory in Alzheimer’s disease rats. At the same time, serum super oxide
dismutase (SOD) and glutathione peroxidase (GSH-Px) activities, and protein carbonyl and malondialdehyde (MDA)
contents were measured to evaluate the antioxidant capacity of COS. Results: Compared with the sham operation control
group, Aβ1–42-exposed rats showed remarkable memory impairment in ethological experiments. In contrast, compared with
the model group, the learning and memory functions of the COS-treated patients were improved. Besides, the rats in the
model group showed significantly lower serum activities of SOD and GSH-Px as well as significantly higher MDA and
protein carbonyl contents compared with the sham operation group. After the intervention of COS, the activities of SOD and
GSH-Px significantly were increased, and MDA and protein carbonyl contents were reduced significantly compared with
the model group. Conclusion: COS has potential therapeutic effect on Aβ1-42-induced cognitive impairment via inhibiting
oxidative stress.

Key words: chitosan oligosaccharide, β-amyloid protein, Alzheimer’s disease, oxidative stress

中图分类号:

Q539

李筱筱，武雪玲，贾世亮，张静，戴雪伶，孙雅煊. 壳寡糖对Amyloid-β1-42致痴呆大鼠的学习记忆及血清抗氧化功能的影响[J]. 食品科学, doi: 10.7506/spkx1002-6630-201701037.

LI Xiaoxiao, WU Xueling, JIA Shiling, ZHANG Jing, DAI Xueling, SUN Yaxuan. Effect of Chitosan Oligosaccharide on Learning and Memory Functions and Serum Antioxidant Status in a Rat Model of Amyloid-β1–42-Induced Alzheimer’s Disease[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201701037.

[1]	黄琳琳，张一敏，朱立贤，梁荣蓉，罗欣，成海建，毛衍伟. 蛋白质氧化和翻译后修饰对肉品质的影响及机制研究进展[J]. 食品科学, 2021, 42(9): 241-247.
[2]	祝敏，靳林，赵浩安，王倩，张锦锦，杨二林，曹炜. 紫穗槐蜂蜜对小鼠急性酒精性胃溃疡的预防作用[J]. 食品科学, 2021, 42(9): 77-84.
[3]	杨成林，黄超，刘娟，黄卫梅，袁志航，易金娥，梁曾恩妮，邬静. 桦木酸对T-2毒素诱导小鼠肝损伤的保护作用[J]. 食品科学, 2021, 42(9): 107-114.
[4]	杨忠敏，沈以红，黄先智，王祖文，丁晓雯. 桑叶生物碱对氧化应激小鼠糖脂代谢异常及肝损伤的改善作用[J]. 食品科学, 2021, 42(7): 156-161.
[5]	黄燕婷，梅丽华，潘海博，覃璐琪，饶川艳，聂梦琳，朱雯君，李全阳. 巴马长寿特征饮食模式对自然衰老小鼠的抗衰老效果[J]. 食品科学, 2021, 42(5): 137-144.
[6]	陈慧, YOSHINORI Katakura, 扈洪波, 尹淑涛, 赵冲. SIRT3多酚激活剂的筛选及其对UVB诱导HaCaT细胞衰老的修复作用[J]. 食品科学, 2021, 42(5): 115-121.
[7]	徐颖，王斌，姜启兴，夏文水，许艳顺. 壳寡糖对酒精诱导的大鼠肠道损伤的干预作用[J]. 食品科学, 2021, 42(3): 158-164.
[8]	何萍萍，郑雅君，郑明静，姜泽东，杜希萍，朱艳冰，倪辉，李清彪. 红毛藻多糖对H2O2诱导的Caco-2细胞氧化损伤的保护作用[J]. 食品科学, 2021, 42(17): 113-120.
[9]	李西栋，孙绍霞，梁亚楠，石塔拉，宓伟. 山楂原花青素和VC联合通过Wnt/β-catenin通路减轻胰岛素抵抗大鼠肝脏氧化应激[J]. 食品科学, 2021, 42(17): 186-192.
[10]	杨诗怡，林巍，杨丽玉，左成，罗勤. CodY在单核细胞增生李斯特菌抗氧化胁迫中的作用[J]. 食品科学, 2021, 42(14): 46-53.
[11]	胡博然，丁建才，曹杨，田颖，国凤华，袁静. (＋)-儿茶素与表没食子儿茶素没食子酸酯对乙醇诱导HepG2细胞脂代谢紊乱及氧化应激的影响[J]. 食品科学, 2021, 42(13): 114-120.
[12]	高华武，王艳，周鹏，叶树，宋航，汪光云，蔡标. 金雀异黄酮通过调控Ca2＋-CaMKIV通路对Aβ25-35诱导海马神经元损伤的保护作用[J]. 食品科学, 2021, 42(13): 121-126.
[13]	华梅，樊美玲，李志满，董丽娜，李珊珊，孙印石. 人参水溶性膳食纤维对大鼠糖脂代谢、氧化应激和肠道健康的影响[J]. 食品科学, 2021, 42(13): 127-135.
[14]	牙甫礼，XIN Yu，张春梅，陈彬林，李玮琪，马永洁. 姜黄素对H2O2诱导血小板凋亡的抑制作用及分子机制[J]. 食品科学, 2021, 42(13): 151-157.
[15]	王新怡，张一敏，梁荣蓉，董鹏程，罗欣，毛衍伟. 过氧化物氧化还原酶6调控肉品嫩度的研究进展[J]. 食品科学, 2021, 42(11): 236-243.

壳寡糖对Amyloid-β1-42致痴呆大鼠的学习记忆及血清抗氧化功能的影响

Effect of Chitosan Oligosaccharide on Learning and Memory Functions and Serum Antioxidant Status in a Rat Model of Amyloid-β1–42-Induced Alzheimer’s Disease

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价