氯吡苯脲处理对采后莲蓬保鲜效果的影响

doi:10.7506/spkx1002-6630-201815033

食品科学 ›› 2018, Vol. 39 ›› Issue (15): 225-232.doi: 10.7506/spkx1002-6630-201815033

氯吡苯脲处理对采后莲蓬保鲜效果的影响

周宏胜1，孙凤杰1,2，罗淑芬1，李鹏霞1,3,*，颜廷才2,*

1.江苏省农业科学院农产品加工研究所，江苏南京 210014；2.沈阳农业大学食品学院，辽宁沈阳 110866； 3.江苏省高效园艺作物遗传改良重点实验室，江苏南京 210014

出版日期:2018-08-15 发布日期:2018-08-15
基金资助:
江苏省农业科技自主创新资金项目（CX（16）1011）

Effect of 1-(2-Chloropyridin-4-yl)-3-phenylurea (CPPU) Treatment on the Postharvest Quality of Lotus Pods

ZHOU Hongsheng1, SUN Fengjie1,2, LUO Shufen1, LI Pengxia1,3,*, YAN Tingcai2,*

1. Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China; 3. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China

Online:2018-08-15 Published:2018-08-15

摘要/Abstract

摘要： 为探索采后莲蓬保鲜新方法，选用‘太空莲36号’为试材，首先以不同质量浓度（2.5、5.0、10.0、15.0、 20.0 mg/L）氯吡苯脲（1-(2-chloropyridin-4-yl)-3-phenylurea，CPPU）对莲蓬进行处理，以莲蓬及莲子感官品质、莲皮色差、莲子蛋白含量为依据，筛选出最佳的莲蓬处理质量浓度；然后，以不作处理（CK0）和清水浸泡（CK1）作对照，在（25±1）℃贮藏条件下，研究了CPPU处理对鲜莲蓬呼吸作用及鲜莲子褐变度、可溶性固形物质量分数和淀粉含量、丙二醛（malondialdehyde，MDA）和过氧化氢（hydrogen peroxide，H2O2）含量、超氧阴离子自由基（superoxide anion radical，O2 －?）生成速率及超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）、过氧化物酶（peroxidase，POD）和多酚氧化酶（polyphenol oxidase，PPO）活力的影响。结果表明：CPPU处理莲蓬的最佳质量浓度为5.0 mg/L，CPPU处理可有效防止莲蓬及莲子褐变，维持莲蓬和莲子较好的表型，并抑制莲蓬呼吸作用，减少莲子可溶性固形物积累和淀粉消耗，减小O2 －?生成速率和H2O2、MDA等的累积，同时与对照组相比，能维持较高的SOD、CAT活力，抑制POD、PPO活力。此外，经CPPU处理后，莲子中CPPU残留量低于国家标准。综上所述，CPPU处理有利于提高采后莲蓬及莲子贮藏品质，是采后鲜莲蓬贮藏保鲜的一种新方法。

关键词: 鲜莲蓬, 氯吡苯脲, 采后保鲜, 品质, 活性氧代谢

Abstract: In order to explore a new method for the preservation of fresh lotus pods, the harvested lotus pods were treated with different concentrations (2.5, 5.0, 10.0, 15.0, and 20.0 mg/L) of 1-(2-chloropyridin-4-yl)-3-phenylurea (CPPU). By analyzing the sensory qualities of lotus pods and seeds, the color difference of lotus seed coats and the protein content of lotus seeds, the optimal concentration of CPPU was selected for the following experiments. Lotus pods without any treatment and those treated with distilled water were used as controls (CK0 and CK1). Then, the effect of CPPU treatment on browning degree, total soluble solids content, starch content, malondialdehyde (MDA) content, hydrogen peroxide (H2O2) content, the rate of superoxide anion radical (O2 -·) generation, super oxide dismutase (SOD) activity, catalase (CAT) activity, peroxidase (POD) activity and polyphenol oxidase (PPO) activity of lotus seeds were investigated during storage at (25 ± 1) ℃. The results showed that the optimal concentration was 5.0 mg/L, and that CPPU treatment effectively prevented the browning of both lotus pods and seeds, maintaining desirable appearance, and inhibited the respiration rate of lotus pods. Moreover, this treatment inhibited the accumulation of soluble solids and starch depletion and lowered the rate of O2 -· generation and the accumulation of H2O2 and MDA. The activities of SOD and CAT were significantly improved, and the activities of POD and PPO were inhibited by CPPU treatment. In addition, the CPPU residue in CPPU-treated lotus seeds was lower than the Chinese national standard limit. Therefore, CPPU treatment is useful for improving postharvest storage quality and delaying senescence of lotus pods and seeds, and will be a promising method for extending the storage life of lotus pods.

Key words: fresh lotus pods, 1-(2-chloropyridin-4-yl)-3-phenylurea (CPPU), postharvest storage, quality, reactive oxygen species metabolism

中图分类号:

TS255.36

周宏胜，孙凤杰，罗淑芬，李鹏霞，颜廷才. 氯吡苯脲处理对采后莲蓬保鲜效果的影响[J]. 食品科学, 2018, 39(15): 225-232.

ZHOU Hongsheng, SUN Fengjie, LUO Shufen, LI Pengxia, YAN Tingcai. Effect of 1-(2-Chloropyridin-4-yl)-3-phenylurea (CPPU) Treatment on the Postharvest Quality of Lotus Pods[J]. FOOD SCIENCE, 2018, 39(15): 225-232.

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氯吡苯脲处理对采后莲蓬保鲜效果的影响

Effect of 1-(2-Chloropyridin-4-yl)-3-phenylurea (CPPU) Treatment on the Postharvest Quality of Lotus Pods

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