食品科学 ›› 2020, Vol. 41 ›› Issue (5): 66-72.doi: 10.7506/spkx1002-6630-20190225-181

• 食品工程 • 上一篇    下一篇

空气放电处理对尖孢镰刀菌生理代谢和细胞膜的影响

刘梦蝶,李彩云,李洁,,严守雷,王清章   

  1. (1.华中农业大学食品科技学院,湖北 武汉 430070;2.环境食品学教育部重点实验室,湖北 武汉 430070;3.湖北省水生蔬菜保鲜加工工程技术研究中心,湖北 武汉 430070)
  • 出版日期:2020-03-15 发布日期:2020-03-23
  • 基金资助:
    中央高校基本科研业务费专项基金项目(2662018JC020)

Effect of Air Discharge Treatment on Physiological Metabolism and Cell Membrane of Fusarium oxysporum

LIU Mengdie, LI Caiyun, LI Jie,, YAN Shoulei, WANG Qingzhang   

  1. (1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2. Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China; 3. Aquatic Vegetable Preservation and Processing Technology Engineering Center of Hubei Province, Wuhan 430070, China)
  • Online:2020-03-15 Published:2020-03-23

摘要: 莲藕因营养丰富易遭受尖孢镰刀菌的侵染而腐败,为了探寻腐败菌的抑制方法,本实验研究了空气放电处理对尖孢镰刀菌的影响,包括空气放电对尖孢镰刀菌物质代谢和能量代谢的影响,并在此基础上,进一步从细胞水平研究了空气放电引起的细胞膜损伤。处理组分别为:臭氧浓度6.87 mg/m3、负离子浓度5×106 ions/cm3以及臭氧6.87 mg/m3+负离子5×106 ions/cm3同时处理,以未经处理的尖孢镰刀菌作为对照组。结果表明:空气放电处理使得尖孢镰刀菌菌丝物质代谢平衡被破坏,糖和蛋白质产生积累;呼吸强度显著减弱(P<0.05),ATP合成显著减少(P<0.05);细胞膜的通透性增大、完整性被破坏,细胞膜流动性显著降低(P<0.05),膜电位显著增加(P<0.05),且臭氧与负离子之间具有协同作用,与单独处理相比,臭氧+负离子处理组更能有效破坏尖孢镰刀菌细胞膜结构。综上,空气放电使得尖孢镰刀菌细胞膜通透性及完整性改变,导致尖孢镰刀菌的分解代谢受到抑制,ATP供应减少,影响细胞活力,从而达到抑菌效果。

关键词: 空气放电, 尖孢镰刀菌, 物质代谢, 能量代谢, 细胞膜

Abstract: Lotus root is apt to be spoiled by Fusarium oxysporum due to its rich nutrition. In order to explore a method to inhibit the spoilage microbe, the effect of air discharge treatment on substance and energy metabolism in Fusarium oxysporum was studied, and we further investigated the cell membrane damage caused by air discharge at the cellular level. Three treatments were set up by using ozone at 6.87 mg/m3 or/and negative ions at 5 × 106 ions/cm3, respectively, and untreated Fusarium oxysporum was regarded as the control group. Results showed that after air discharge treatment, the metabolic balance of Fusarium oxysporum mycelium was destroyed, and consequently sugar and protein accumulated. Respiratory intensity and ATP synthesis decreased significantly (P < 0.05). Moreover, the cell membrane permeability increased, the integrity was destroyed, the fluidity significantly decreased (P < 0.05), and the membrane potential increased significantly (P < 0.05). Compared with the single treatments, the combined treatment was more effective in destroying cell membrane structure. In conclusion, air discharge exerted bacteriostatic effect by changing the cell membrane permeability and integrity of Fusarium oxysporum, and inhibiting catabolism, consequently decreasing ATP supply and finally affecting cell viability.

Key words: air discharge, Fusarium oxysporum, substance metabolism, energy metabolism, cell membrane

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