食品科学 ›› 2020, Vol. 41 ›› Issue (14): 294-299.doi: 10.7506/spkx1002-6630-20190326-342

• 安全检测 • 上一篇    下一篇

树枝状表面增强拉曼散射基底制备及孔雀石绿痕量检测

赵静晨,黄丹丹,朱树华   

  1. (山东农业大学化学与材料科学学院,山东 泰安 271018)
  • 发布日期:2020-07-29
  • 基金资助:
    国家自然科学基金面上项目(31770724);国家自然科学基金青年科学基金项目(31800581)

Preparation and Application of Dendritic Surface-Enhanced Raman Scattering Substrates in the Detection of Trace Malachite Green

ZHAO Jingchen, HUANG Dandan, ZHU Shuhua   

  1. (College of Chemistry and Material Science, Shandong Agricultural University, Tai’an 271018, China)
  • Published:2020-07-29

摘要: 在表面活性剂十二烷基甲基溴化哌啶(N-methyl-N-dodecylpiperidinium bromide,C12PDB)溶液中以抗坏血酸为还原剂还原氯金酸(HAuCl4),可快速合成具有树枝状结构的金纳米粒子(gold nanoparticles,Au NPs)。透射电子显微镜结果显示得到的树枝状Au NPs具有各向异性生长的均匀对称结构,大小为3.5~4 μm。此树枝状Au NPs具有优异的拉曼活性,作为表面增强拉曼光谱(surface-enhanced Raman spectroscopy,SERS)基底检测罗丹明6G(rhodamine 6G,R6G)表现出了较高的灵敏度和良好的检测重复性,可测得R6G溶液最低浓度为3×10-9 mol/L,计算得增强因子约为105,其检测线性范围为3×10-9~3×10-7 mol/L,10-6 mol/L R6G的10 次重复检测结果中各特征峰强度的相对标准偏差均低于10%。以树枝状Au NPs作为SERS基底对溶液中孔雀石绿的检出限为1×10-8 mol/L,并可用于鲫鱼肉中孔雀石绿的快速检测,样品加标回收率为81.6%~102.1%。

关键词: 十二烷基甲基溴化哌啶;树枝状金纳米粒子;表面增强拉曼光谱;孔雀石绿

Abstract: Dendritic gold nanoparticles (Au NPs) were synthesized by reducing chloroauric acid (HAuCl4) with ascorbic acid in an aqueous solution of N-methyl-N-dodecylpiperidinium bromide (C12PDB). Transmission electron microscopic (TEM) images showed that the dendritic Au NPs, with good symmetry and multilevel branches, had a diameter of 3.5–4 μm. When surface-enhanced Raman substrates (SERS) were used to detect rhodamine 6G (R6G), these Au NPs showed excellent enhancement effect with an enhancement factor of about 105. These SERS substrates showed high sensitivity with a minimum detectable concentration of R6G as low as 3 × 10-9 mol/L. The SERS substrates based on these dendritic Au NPs also showed good signal repeatability with relative standard deviation (RSD) lower than 10% (n = 10). The dendritic Au NPs substrates afforded limits of detection (LOD) around 1 × 10-8 mol/L for aqueous malachite green (MG) solutions. These Au NPs were successfully applied to rapidly detect MG in Carassius auratus samples. The recoveries of MG were 81.6%–102.1% in the spiked samples.

Key words: N-methyl-N-dodecylpiperidinium bromide; dendritic gold nanoparticles; surface-enhanced Raman spectroscopy; malachite green

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