食品科学 ›› 2020, Vol. 41 ›› Issue (18): 134-139.doi: 10.7506/spkx1002-6630-20190802-037

• 生物工程 • 上一篇    下一篇

基于C14-HSL探讨阪崎肠杆菌中信号分子与其生长机制关系

闫晓彤,罗小娟,潘洁茹,叶海梅,林侃,李长城,方婷   

  1. (1.福建农林大学食品科学学院,福建?福州 350002;2.福州市疾病控制预防中心,福建?福州 350004)
  • 出版日期:2020-09-25 发布日期:2020-09-18
  • 基金资助:
    国家自然科学基金青年科学基金项目(31401597;31601393);福建农林大学国际合作项目(KXb16012A); 2018年度福州市卫生计生科研创新团队培育项目(2018-S-wt7);福州市“十三五”海洋经济创新发展示范项目; 福建农林大学科技创新专项基金立项项目(KFA17599A;KFA18058A)

Relationship between the Signal Molecule N-Tetradecanoyl-L-homoserine lactone in Cnterobacter sakazakii and Its Growth

YAN Xiaotong, LUO Xiaojuan, PAN Jieru, YE Haimei, LIN Kan, LI Changcheng, FANG Ting   

  1. (1. School of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2. Fuzhou Center for Disease Control and Prevention, Fuzhou 350004, China)
  • Online:2020-09-25 Published:2020-09-18

摘要: 研究37?℃阪崎克罗诺肠杆菌(Cronobacter sakazakii)CICC21550在生长过程中信号分子C14-HSL浓度变化的拟合曲线,结合细菌生长的宏观模型,进一步从微观角度探究C. sakazakii的生长机制。结果表明:C. sakazakii CICC21550在对数生长初期已检出C14-HSL信号分子,在细菌生长的稳定期达最大值,随后呈逐渐下降趋势,具有明显的密度依赖性。C14-HSL信号分子的最大浓度Ymax为8.535,最大生成速率μmax为0.825。

关键词: 阪崎克罗诺肠杆菌;信号分子;预测模型;IPMP

Abstract: In this paper, the change in the concentration of the signal molecule N-tetradecanoyl-L-homoserine lactone (C14-HSL) during the growth process of Cnterobacter sakazakii CICC21550 at 37 ℃ was fitted to a curve. Based on the macroscopic model of bacterial growth, the growth mechanism of C. sakazakii CICC21550 was further explored from the microscopic perspective. The results showed that C14-HSL was detected in C. akazakii CICC21550 at the early logarithmic phase of growth, which reached the maximum value in the stationary period of bacterial growth, and then showed a gradually declining trend and it was in an obvious density-dependence manner. The maximum concentration Ymax of C14-HSL was 8.535 and the maximum rate of formation μmax was 0.825.

Key words: Cronobacter sakazakii; signal molecules; prediction model; Integrated Pathogen Modeling Program (IPMP)

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