食品科学 ›› 2020, Vol. 41 ›› Issue (20): 278-284.doi: 10.7506/spkx1002-6630-20190822-224

• 工艺技术 • 上一篇    下一篇

基于生物信息学定向制备柠条籽蛋白抗氧化肽的工艺优化

頡宇,胡锦灵,赵宏飞,张柏林   

  1. (北京林业大学生物科学与技术学院,北京 100083)
  • 出版日期:2020-10-25 发布日期:2020-10-23

Optimization of Bioinformatics-Based Directional Preparation of Antioxidant Peptide from Caragana Seed Protein

JIE Yu, HU Jinling, ZHAO Hongfei, ZHANG Bolin   

  1. (College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China)
  • Online:2020-10-25 Published:2020-10-23

摘要: 筛选谷蛋白作为柠条籽蛋白生物信息模板,基于BIOPEP数据库,选用木瓜蛋白酶、胃蛋白酶、胰蛋白酶和V-8蛋白酶进行虚拟酶解,通过验证水解度和亚油酸氧化抑制率,确定木瓜蛋白酶+10% V-8蛋白酶作为水解柠条籽蛋白优选酶。经单因素试验和正交试验设计,获得柠条籽蛋白优化酶解工艺为水解温度45 ℃、pH 5.0、水解时间2 h、酶添加量1%,采用质谱鉴定富含活性片段的多肽序列,其中,柠条籽肽LDEPDPL的亚油酸氧化抑制率达到50.3%。因此,借助计算机辅助和酶法水解相结合的方法,为定向制备抗氧化肽提供了新的研究策略。

关键词: 柠条籽蛋白;抗氧化肽;生物信息学;油脂氧化

Abstract: In order to determine the most suitable enzyme to prepare antioxidant peptide from Caragana seed protein, gluten was chosen as the bioinformatic template of Caragana seed protein for simulated hydrolysis by four different enzymes including papain, pepsin, trypsin and V-8 protease based on the BIOPEP database. Based on degree of hydrolysis and linoleic acid oxidation inhibitory rate, papain plus 10% V-8 protease was regarded as the best enzyme for hydrolyzing Caragana seed protein. Using one-factor-at-a-time and orthogonal array design methods, the optimal hydrolysis parameters were obtained as follows: temperature 45 ℃, hydrolysis time 2 h, pH 5.0, and enzyme dosage 1%. As a result, six antioxidant peptides were obtained whose sequences were identified by mass spectrometry. The inhibition rate of LDEPDPL against linoleic acid oxidation was 50.3%. In conclusion, the computer-assisted enzymatic hydrolysis provides a novel strategy for directional preparation of antioxidant peptide from Caragana seed protein.

Key words: Caragana seed protein; antioxidant peptide; bioinformatics; lipid oxidation

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