大黄鱼卵分离蛋白乳液的构筑及其体外消化规律

doi:10.7506/spkx1002-6630-20190914-167

食品科学 ›› 2020, Vol. 41 ›› Issue (18): 8-13.doi: 10.7506/spkx1002-6630-20190914-167

大黄鱼卵分离蛋白乳液的构筑及其体外消化规律

王笑涵，姜卉，吴海涛，张志慧，崔浩哲，于佳卉，杨贺，唐越

（大连工业大学食品学院，国家海洋食品工程技术研究中心，辽宁?大连 116034）

出版日期:2020-09-25 发布日期:2020-09-18
基金资助:
国家自然科学基金青年科学基金项目（31901687）；辽宁省博士科研启动基金计划项目（201601269）

Construction and In Vitro Digestion Properties of Pseudosciaena crocea Roe Protein Isolate-Stabilized Emulsion

WANG Xiaohan, JIANG Hui, WU Haitao, ZHANG Zhihui, CUI Haozhe, YU Jiahui, YANG He, TANG Yue

(National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)

Online:2020-09-25 Published:2020-09-18

摘要/Abstract

摘要： 为了提高β-胡萝卜素的溶解性以及生物利用度，以大黄鱼卵分离蛋白（Pseudosciaena crocea roe protein isolate，PRPI）为乳化剂构建稳定的PRPI-β-胡萝卜素水包油乳液体系，考察不同贮存时间下乳液体系稳定性的变化；通过体外模拟消化明确其消化过程及吸收能力。结果表明，在贮存14?d后，乳液体系的平均粒径及外观形态均无显著改变；模拟口腔消化对乳液体系微观结构的状态影响较小，但经胃消化后，乳液体系发生水解，液滴聚集，平均粒径由（255.46±7.41）?nm增大至（9?749.25±406.66）?nm（P＜0.05）；经小肠消化后，液滴聚集减弱，平均粒径减小至（3?654.36±527.91）?nm（P＜0.05），仍有较高的β-胡萝卜素保留率（81.64±2.72）%和生物利用度（93.13±3.59）%。因此，以PRPI为乳化剂不仅可以提高乳液输送体系的稳定性，还可以提高β-胡萝卜素的生物利用度。

关键词: 大黄鱼卵分离蛋白；β-胡萝卜素；乳液体系；体外消化；生物利用度

Abstract: In order to improve the solubility and bioavailability of β-carotene, a stable β-carotene-loaded oil-in-water (O/W) emulsion system was constructed using Pseudosciaena crocea roe protein isolate (PRPI) as an emulsifier. The stability of the emulsion system with different storage times was investigated. The digestion process and absorption capacity were determined by in vitro simulated digestion. The results showed that the average particle size and appearance of the emulsion system did not change significantly after 14 days of storage. Simulated oral digestion had little effects on the microstructure of the emulsion system. However, during simulated gastric digestion, the emulsion system was hydrolyzed and the droplets were aggregated, the average particle size increasing from (255.46 ± 7.41) nm to (9 749.25 ± 406.66) nm (P < 0.05). After simulated intestinal digestion, the degree of droplet aggregation decreased significantly, the average particle size decreasing to (3 654.36 ± 527.91) nm (P < 0.05), but the retention rate and bioavailability of β-carotene remained at high levels of (81.64 ± 2.72)% and (93.13 ± 3.59)%, respectively. Therefore, PRPI could be used as an emulsifier to construct an emulsion delivery system with improved stability for improved β-carotene bioavailability.

Key words: Pseudosciaena crocea roe protein isolate; β-carotene; emulsion system; in vitro digestion; bioavailability

中图分类号:

TS254

王笑涵，姜卉，吴海涛，张志慧，崔浩哲，于佳卉，杨贺，唐越. 大黄鱼卵分离蛋白乳液的构筑及其体外消化规律[J]. 食品科学, 2020, 41(18): 8-13.

WANG Xiaohan, JIANG Hui, WU Haitao, ZHANG Zhihui, CUI Haozhe, YU Jiahui, YANG He, TANG Yue. Construction and In Vitro Digestion Properties of Pseudosciaena crocea Roe Protein Isolate-Stabilized Emulsion[J]. FOOD SCIENCE, 2020, 41(18): 8-13.

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大黄鱼卵分离蛋白乳液的构筑及其体外消化规律

Construction and In Vitro Digestion Properties of Pseudosciaena crocea Roe Protein Isolate-Stabilized Emulsion

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