食品科学 ›› 2021, Vol. 42 ›› Issue (1): 67-72.doi: 10.7506/spkx1002-6630-20191202-032

• 基础研究 • 上一篇    下一篇

罗伊氏菌素与儿茶素协同对变异链球菌的抑制作用

王思彤,张彤,金曼,曲国强,孙雪婷,付恒芳,刘丽波,李春   

  1. (1.东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江 哈尔滨 150030;2.黑龙江省绿色食品科学研究院,黑龙江 哈尔滨 150028;3.国家乳制品质量监督检验中心理化室,黑龙江 哈尔滨 150028)
  • 发布日期:2021-01-18
  • 基金资助:
    “十三五”国家重点研发计划重点专项(2018YFD0502404);黑龙江省科学基金项目(C2018022); 东北农业大学“学术骨干计划”项目(18XG27)

Synergistic Inhibition Mechanism of Reuterin and Catechin against Streptococcus mutans

WANG Sitong, ZHANG Tong, JIN Man, QU Guoqiang, SUN Xueting, FU Hengfang, LIU Libo, LI Chun   

  1. (1. Key Laboratory of Dairy Science, Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Institute of Green Food Science, Harbin 150028, China; 3. Physical and Chemical Office, National Dairy Product Quality Supervision and Inspection Center, Harbin 150028, China)
  • Published:2021-01-18

摘要: 为探讨儿茶素与罗伊氏菌素(主要抗菌成分为3-羟基丙醛(3-hydroxypropionaldehyde,3-HPA))对诱发龋齿的变异链球菌的抑制作用及其机制,本研究测定了罗伊氏菌素与儿茶素单独使用和联合作用时对变异链球菌的最小抑菌浓度(minimum inhibitory concentration,MIC),经计算分级抑制浓度指数得知,当儿茶素(1/8 MIC)和3-HPA(1/4 MIC)联合使用时具有协同作用。测定生物膜含量、胞外多糖(extracellular polysaccharide,EPS)含量和有机酸含量3 个指标时发现,协同组使各指标含量显著减小(P<0.05),且效果优于单一组。扫描电子显微镜结果显示,协同组生物膜结构改变明显、膜厚度降低、菌体数量减少。综上可看出,儿茶素和3-HPA协同使用能够抑制菌体的聚集与黏附、参与底物代谢,提高酸度、延缓生物膜生长成熟,可作为新型抑菌剂应用于防龋产品,为今后开发天然、安全的防龋产品提供理论依据。

关键词: 罗伊氏菌素;儿茶素;协同作用;变异链球菌;生物膜

Abstract: In order to investigate the inhibitory effect of catechin and reuterin (3-hydroxypropionaldehyde (3-HPA)) on the caries pathogen Streptococcus mutans and to dissect the underlying mechanisms, the minimum inhibitory concentrations of reuterin and catechin alone and in combination were determined in the present study. By calculating the fractional inhibitory concentration index (FICI), it was found that catechin (1/8 MIC) and 3-HPA (1/4 MIC) had a synergistic effect when used together. The combination of the two drug resulted in a significant reduction in the contents of biofilm, extracellular polysaccharide (EPS) and organic acid (P < 0.05), more effective than either drug alone. Scanning electron microscopic results showed that the biofilm structure in the combination treatment group significantly changed compared to either individual treatment group, accompanied by a decrease in biofilm thickness and the number of bacterial cells. Collectively, the above results demonstrated that the combination treatment of catechin and 3-HPA could inhibit bacterial aggregation and adhesion, impede bacterial cells from being involved in substrate metabolism, increase acidity and delay biofilm?growth?and?maturation, and thus could be used as a novel antibacterial agent against caries. This study will provide a theoretical basis for the development of natural and safe anti-caries products.

Key words: reuterin; catechin; synergistic effect; Streptococcus mutans; biofilm

中图分类号: