食品科学 ›› 2020, Vol. 41 ›› Issue (24): 125-130.doi: 10.7506/spkx1002-6630-20191222-254

• 成分分析 • 上一篇    下一篇

矿物元素结合稳定同位素的中华绒螯蟹产地溯源

张政权,黄冬梅,蔡友琼,孟宪菁,史永富,孔聪,黄宣运,汤云瑜,张璇,杨光昕   

  1. (1.上海海洋大学食品学院,上海 201306;2.中国水产科学研究院东海水产研究所,农业农村部远洋与极地渔业创新重点实验室,上海 200090;3.赛默飞世尔科技(中国)有限公司,上海 201206)
  • 出版日期:2020-12-25 发布日期:2020-12-28
  • 基金资助:
    国家自然科学基金青年科学基金项目(31501572)

Geographical Origin Traceability of Chinese Mitten Crabs Based on Mineral Elements and Stable Isotopes

ZHANG Zhengquan, HUANG Dongmei, CAI Youqiong, MENG Xianjing, SHI Yongfu, KONG Cong, HUANG Xuanyun, TANG Yunyu, ZHANG Xuan, YANG Guangxin   

  1. (1. College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China;2. Key Laboratory of Oceanic and Polar Fisheries Innovation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China; 3. Thermo Fisher Scientific (China) Co. Ltd., Shanghai 201206, China)
  • Online:2020-12-25 Published:2020-12-28

摘要: 为了探寻矿物元素和稳定同位素在中华绒螯蟹产地溯源中的应用前景,建立一种中华绒螯蟹的产地溯源体系。采用微波消解-原子吸收光谱法和稳定同位素比率质谱仪分别测定崇明、阳澄湖和兴化3 个地区中华绒螯蟹肌肉组织中K、Ca、Na、Mg、Mn、Zn、Cu、Fe 8 种矿物元素的含量和C、N稳定同位素丰度,结合多元统计分析方法建立对3 个地区的中华绒螯蟹产地判别模型。线性判别分析对崇明、阳澄湖和兴化3 个地区样品的判别正确率分别为100%、95%和100%,初始总体判别正确率为98.3%,交叉验证判别正确率为98.3%,主成分分析最终筛选出4 个主要成分,累计方差贡献率为74.8%,采用第1、2和4主成分建立坐标系,绘制3 个地区的样品在坐标系中的散点图,3 个地区的样品分布在坐标系中3 个不同的区域。证明矿物元素结合稳定同位素可以对长江水系不同地区的中华绒螯蟹进行区分。

关键词: 中华绒螯蟹;产地溯源;矿物元素;稳定同位素

Abstract: This study was undertaken in order to explore the potential of mineral elements and stable isotopes for the geographical origin traceability of Eriocheir sinensis. Microwave digestion-atomic absorption spectrometry and stable isotope ratio mass spectrometry were used to determine the contents of eight mineral elements (K, Ca, Na, Mg, Mn, Zn, Cu and Fe) and the abundances of C and N stable isotopes in the muscle tissue of E. sinensis from Chongming of Shanghai, and Yangcheng Lake and Xinghua of Jiangsu. By using multivariate statistical analysis, we established a discriminant model for distinguishing the three geographical origins from each other. The correct discrimination rates for samples from Chongming, Yangcheng Lake and Xinghua were 100%, 95% and 100%, respectively. The initial overall discriminant accuracy was 98.3%, and the cross-validation accuracy was 98.3%. Four principal components were selected by principal component analysis (PCA), cumulatively contributing to 74.8% of the total variance. In the three-dimensional PCA scatter plot of PC1 versus PC2 versus PC4, the three samples were clearly separated. It is proved that mineral elements combined with stable isotopes can distinguish Chinese mitten crabs from different regions of the Yangtze River.

Key words: Chinese mitten crab; geographical origin traceability; mineral elements; stable isotopes

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