离子色谱串联三重四极杆质谱测定婴幼儿配方乳粉和牛乳中的肌醇

doi:10.7506/spkx1002-6630-20191225-298

食品科学 ›› 2020, Vol. 41 ›› Issue (24): 185-189.doi: 10.7506/spkx1002-6630-20191225-298

离子色谱串联三重四极杆质谱测定婴幼儿配方乳粉和牛乳中的肌醇

王岩，王娟，吴春敏，张春林，王东，高镯，张雷雷，张岩

（河北省食品检验研究院，河北石家庄 050000）

出版日期:2020-12-25 发布日期:2020-12-28
基金资助:
“十三五”国家重点研发计划重点专项（2019YFC1606406）；河北省科技计划项目（19225503D）；国家市场监管总局技术保障专项（2019YJ009）

Determination of Inositol in Infant Formula and Milk by Ion Chromatography-Tandem Triple Quadrupole Mass Spectrometry

WANG Yan, WANG Juan, WU Chunmin, ZHANG Chunlin, WANG Dong, GAO Zhuo, ZHANG Leilei, ZHANG Yan

(Hebei Food Inspection and Research Institute, Shijiazhuang 050000, China)

Online:2020-12-25 Published:2020-12-28

摘要/Abstract

摘要： 采用离子色谱串联三重四极杆质谱技术测定婴幼儿配方乳粉和牛乳中的肌醇。样品通过溶解提取、调节pH值、离心过滤等前处理过程，以糖分析Dionex CarboPac MA1型色谱柱（2.0 mm×250 mm，7.5 μm）作为分析柱，以在线淋洗液自动发生器配制的KOH为淋洗液进行梯度洗脱，采用电喷雾离子源，负离子模式进行检测。结果表明：婴幼儿配方乳粉和牛乳中肌醇在质量浓度0.01～3.00 μg/mL范围内线性良好，相关系数r不小于0.999，方法检出限和定量限分别为0.023 mg/100 g和0.076 mg/100 g，均低于国家标准，加标回收率在90.3%～109.5%之间，相对标准偏差在0.7%～9.1%之间（n=6）。本方法操作简单、结果稳定好、灵敏度高，适用于婴幼儿配方乳粉和牛乳中肌醇的测定。

关键词: 离子色谱串联三重四极杆质谱；婴幼儿配方乳粉；牛乳；肌醇

Abstract: In this study, inositol contents in infant formula and milk were determined using ion chromatography-tandem triple quadrupole mass spectrometry. The sample pretreatment procedure included extracted by dissolution, extraction, pH adjustment, centrifugation and filtration. The analysis was performed on a Dionex CarboPac MA1 column (2.0 mm × 250 mm, 7.5 μm) using gradient elution with KOH generated online with an automatic eluent generator as eluent. Electrospray ionization was operated in the negative ion mode to detect the analyte. The results showed that good linearity with correlation coefficient (r) ≥ 0.999 was observed for inositol in infant formula and milk within the concentration range of 0.01–3.00 μg/mL. The limit of detection (LOD) and limit of quantitation (LOQ) of the method were 0.023 and 0.076 mg/100 g, respectively, both lower than those specified in the national standard of China. The recoveries of spiked samples were between 90.3% and 109.5% with relative standard deviations (RSDs) of 0.7%–9.1% (n = 6). The method was simple to operate, stable and sensitive and could be suitable for the determination of inositol in infant formula and milk.

Key words: ion chromatography-tandem triple quadrupole mass spectrometry; infant formula; milk; inositol

中图分类号:

TS202.1

王岩，王娟，吴春敏，张春林，王东，高镯，张雷雷，张岩. 离子色谱串联三重四极杆质谱测定婴幼儿配方乳粉和牛乳中的肌醇[J]. 食品科学, 2020, 41(24): 185-189.

WANG Yan, WANG Juan, WU Chunmin, ZHANG Chunlin, WANG Dong, GAO Zhuo, ZHANG Leilei, ZHANG Yan. Determination of Inositol in Infant Formula and Milk by Ion Chromatography-Tandem Triple Quadrupole Mass Spectrometry[J]. FOOD SCIENCE, 2020, 41(24): 185-189.

[1]	郑炯. 不同解冻方式对速冻方竹笋品质的影响[J]. 食品科学, 0, (): 0-0.
[2]	韦铮贺燕黄先智沈以红丁晓雯. 茶多糖-茶多酚对小鼠肠道氧化应激的改善与作用机制[J]. 食品科学, 0, (): 0-0.
[3]	贾蕾向极钎殷红清何慧侯焘. 生物活性硒肽的研究进展[J]. , 0, (): 0-0.
[4]	周秀雯吴浩陈海泉颜治靳保辉谢丽琪赵燕赵超敏陈辉潘家荣. 基于矿物元素指纹差异的榴莲产地甄别[J]. , 0, (): 0-0.
[5]	王斌陈逸伦夏文水. 壳寡糖对酒精诱导的新生大鼠脑组织氧化应激损伤和凋亡因子的影响[J]. 食品科学, 0, (): 0-0.
[6]	李祥，解玉丽，贾园园，张闪，王红艳，刘先吏，罗湘艳，唐存多. 基于基因组挖掘技术的新型谷氨酸脱羧酶基因挖掘及表达鉴定[J]. 食品科学, 2021, 42(10): 79-85.
[7]	刘柯欣林松毅胡胜杰张秀敏孙娜. 南极磷虾蛋白营养与功能特性及食用安全性研究进展[J]. 食品科学, 0, (): 0-0.
[8]	王佳玉，陈凤莲，吴迪，汤晓智. 谷氨酰胺转氨酶对全麦面团特性及微观结构的影响[J]. 食品科学, 2021, 42(4): 51-57.
[9]	王佳玉陈凤莲吴迪汤晓智. 谷氨酰胺转氨酶对全麦面团特性及微观结构的影响[J]. , 0, (): 0-0.
[10]	贾园园，李祥，张振华，张闪，杨露露，唐存多. 重组大肠杆菌全细胞催化D,L-扁桃酸对映选择性制备L-苯甘氨酸[J]. 食品科学, 2021, 42(2): 83-89.
[11]	王岩. 离子色谱串联三重四级杆质谱测定婴幼儿配方乳粉和牛乳中的肌醇[J]. , 0, (): 0-0.
[12]	李昭锋，曹潇，朱杰，陈思谦，李琳. 细菌纤维素在植物细胞壁结构与功能研究中的应用及进展[J]. 食品科学, 2020, 41(19): 263-271.
[13]	张欣昕张福金刘广华张尧莎娜连海飞. 基于矿质元素和稳定同位素的马铃薯产地溯源技术[J]. , 0, (): 0-0.
[14]	张欣昕，张福金，刘广华，张尧，王雪娇，莎娜，连海飞. 基于矿质元素和稳定同位素的马铃薯产地溯源技术[J]. 食品科学, 2020, 41(18): 296-302.
[15]	牛丽影, 李大婧, 刘春泉, 宋江峰, 陈计峦, 陈艳萍, 袁建华. 鲜食玉米发育过程中游离糖和氨基酸变化[J]. 食品科学, 0, (): 165-172.

离子色谱串联三重四极杆质谱测定婴幼儿配方乳粉和牛乳中的肌醇

Determination of Inositol in Infant Formula and Milk by Ion Chromatography-Tandem Triple Quadrupole Mass Spectrometry

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价