基于斑马鱼模型研究鱿鱼生殖腺磷脂抗MPTP诱发帕金森病的活性

doi:10.7506/spkx1002-6630-20200228-321

食品科学 ›› 2021, Vol. 42 ›› Issue (5): 122-128.doi: 10.7506/spkx1002-6630-20200228-321

基于斑马鱼模型研究鱿鱼生殖腺磷脂抗MPTP诱发帕金森病的活性

董俊凡，宋扬，季秀娜，李晓彬，刘可春，靳梦

（1.齐鲁工业大学（山东省科学院）生物研究所，山东济南 250103；2.齐鲁工业大学（山东省科学院）生物工程学院，山东济南 250353）

出版日期:2021-03-15 发布日期:2021-03-29
基金资助:
国家自然科学基金青年科学基金项目（81802629）；山东省科学院国际科技合作项目（2019GHZD10）；山东省重点研发计划项目（2019GSF107053）；齐鲁工业大学（山东省科学院）国际合作研究专项资金项目（QLUTGJHZ2018010）

Protective Effect of Squid Gonadal Phospholipids on MPTP-Induced Zebrafish Model of Parkinson’s Disease

DONG Junfan, SONG Yang, JI Xiuna, LI Xiaobin, LIU Kechun, JIN Meng

(1. Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China;2. School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China)

Online:2021-03-15 Published:2021-03-29

摘要/Abstract

摘要： 目的：探究鱿鱼生殖腺磷脂是否具有抗1-甲基-4-苯基-1,2,3,6-四氢吡啶盐酸盐（1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine，MPTP）诱发帕金森病的活性。方法：将受精后1 d（day post fertilization，dpf）的斑马鱼胚胎脱膜，设置空白对照组、50 μmol/L MPTP处理组、50 μmol/L MPTP与不同质量浓度（3、10、20 μg/mL）鱿鱼生殖腺磷脂共处理组。4 dpf时用荧光显微镜观察并记录各实验组转基因斑马鱼Vmat:GFP的多巴胺神经元发育情况和转基因斑马鱼Fli1:GFP的脑部血管发育情况；5 dpf时用Zebrabox斑马鱼行为分析仪分析野生型斑马鱼AB的行为变化，并通过实时荧光定量聚合酶链式反应检测帕金森病相关基因的表达情况，包括α-突触核蛋白、E3泛素连接酶、PTEN诱导假定激酶1、酪氨酸羟化酶编码基因。结果：与空白对照组比较，MPTP处理组斑马鱼多巴胺神经元缺失、脑部血管损伤，出现帕金森状行为，帕金森病相关基因表达异常；与MPTP处理组比较，不同质量浓度鱿鱼生殖腺磷脂与MPTP共处理组斑马鱼表现出多巴胺神经元缺失比例、脑部血管损伤程度降低，帕金森状行为缓解，相关基因异常表达趋于正常。

关键词: 鱿鱼生殖腺磷脂；1-甲基-4-苯基-1,2,3,6-四氢吡啶盐酸盐；斑马鱼；帕金森病

Abstract: Purpose: To explore whether squid gonadal phospholipids have an inhibitory effect on Parkinson’s disease (PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Methods: Zebrafish embryos at 1 day post fertilization (dpf) were dechorionated and then divided into different groups including control, 50 μmol/L MPTP, and 50 μmol/L MPTP combined with different concentrations (3, 10 and 20 μg/mL) of squid gonadal phospholipids. Transgenci zebrafish Vmat:GFP and Fli1:GFP at 4 dpf were used to observe and record the development of dopaminergic neurons and brain blood vessels, respectively. At 5 dpf, we utilized the Zebrabox video-tracking system to assay the behavioral changes of wild-type AB zebrafish. Meanwhile, we analyzed the expression of PD-related genes including those encoding alpha-synuclein (α-syn), E3 ubiquitin protein ligase (parkin), PTEN-induced putative kinase 1 (pink1), and tyrosine hydroxylase (th) by real-time fluorescence quantitative polymerase chain reaction. Results: Compared with the control group, zebrafish treated with MPTP exhibited loss of dopaminergic neurons, injured blood vessels in the brain, PD-like behavior, and abnormal expression of PD-related genes. In comparison to the MPTP treatment group, MPTP combined with squid gonadal phospholipids showed a decrease in the percentage dopamine neuron loss, and relieved blood vessel damage in the brain, PD-like behavior, and the abnormal expression of PD-related genes.

Key words: squid gonadal phospholipid; 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; zebrafish; Parkinson’s disease

中图分类号:

R746.1

董俊凡，宋扬，季秀娜，李晓彬，刘可春，靳梦. 基于斑马鱼模型研究鱿鱼生殖腺磷脂抗MPTP诱发帕金森病的活性[J]. 食品科学, 2021, 42(5): 122-128.

DONG Junfan, SONG Yang, JI Xiuna, LI Xiaobin, LIU Kechun, JIN Meng. Protective Effect of Squid Gonadal Phospholipids on MPTP-Induced Zebrafish Model of Parkinson’s Disease[J]. FOOD SCIENCE, 2021, 42(5): 122-128.

基于斑马鱼模型研究鱿鱼生殖腺磷脂抗MPTP诱发帕金森病的活性

Protective Effect of Squid Gonadal Phospholipids on MPTP-Induced Zebrafish Model of Parkinson’s Disease

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