食品科学 ›› 2021, Vol. 42 ›› Issue (14): 54-61.doi: 10.7506/spkx1002-6630-20200509-092

• 生物工程 • 上一篇    下一篇

不同寡营养条件下副溶血性弧菌的生长异质性

张布克,史俊,潘迎捷,赵勇,刘海泉   

  1. (1.上海海洋大学食品学院,上海 201306;2.上海水产品加工及贮藏工程技术研究中心,上海 201306;3.农业农村部水产品贮藏保鲜质量安全风险评估实验室(上海),上海 201306;4.上海海洋大学食品热加工工程技术研究中心,上海 201306)
  • 发布日期:2021-07-27
  • 基金资助:
    国家自然科学基金面上项目(31671779;31972188); “十三五”国家重点研发计划重点专项(2018YFC1602205;2018YFC160220); 上海市科委上海市“科技创新行动计划”农业领域项目(19391901600); 上海市科技兴农项目(沪农科推字2017第4-4号);上海市教育委员会科研创新计划项目(2017-01-07-00-10-E00056)

Growth Heterogeneity of Vibrio parahaemolyticus under Different Oligotrophic Conditions

ZHANG Buke, SHI Jun, PAN Yingjie, ZHAO Yong, LIU Haiquan   

  1. (1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China;2. Shanghai Engineering Research Center of Aquatic-product Processing & Preservation, Shanghai 201306, China; 3. Laboratory of Quality & Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Ministry of Agriculture and Rural Affairs, Shanghai 201306, China; 4. Engineering Research Center of Food Thermal-processing Technology, Shanghai Ocean University, Shanghai 201306, China)
  • Published:2021-07-27

摘要: 为更准确地探讨副溶血性弧菌(Vibrio parahaemolyticus,Vp)菌株在寡营养条件(100%、75%、50%和25%标准TSB+(3% NaCl)占比的培养基稀释液)下的生长异质性对食品安全风险的影响,本实验选取20 株分离自淡水和海水养殖虾中的Vp菌株,通过对其在不同寡营养条件下的生长数据进行拟合,得到20 株不同来源Vp菌株在不同寡营养条件下的一级生长模型,并对二级生长参数进行分析。结果表明:修正Gompertz一级模型对20 株不同来源的Vp菌株在不同寡营养条件下的生长数据拟合良好,拟合相关系数R2为0.791~0.997。不同来源Vp菌株在同种营养浓度下最大比生长速率无显著统计学差异(P>0.05),不同寡营养条件下Vp菌株生长参数不同,与其他研究相比得出初始菌液接种量不同也会造成菌株的生长异质性。综上,不同寡营养条件下Vp菌株有较大的生长异质性,研究这种差异有助于更精准的食品安全风险评估和更科学的食源性致病菌所致疾病防控。

关键词: 副溶血性弧菌;寡营养条件;生长异质性;生长模型

Abstract: In order to study the effect of growth heterogeneity of Vibrio parahaemolyticus (Vp) on food safety risk under oligotrophic conditions: different dilutions (100%, 75%, 50% and 25%) of standard TSB+ (3% NaCl) medium, 20 Vp strains isolated from prawns and shrimps were selected. A first-order growth model was fitted for each strain, and second-order growth parameters were analyzed. The results proved that the modified Gompertz first-order model was fitted to the growth data of the Vp strains well, with correlation coefficient R2 of 0.791–0.997. There was no significant difference in the maximum specific growth rate (P > 0.05) under the same nutrient concentration among Vp strains from different sources. The growth parameters of Vp strains were different under different oligotrophic conditions. Through comparison of the present results with those of previous studies, we found that different initial inoculation amounts of Vp strains would also cause the growth heterogeneity of the strains. In summary, Vp strains had great growth heterogeneity under different oligotrophic conditions. The results of this study will contribute to more accurate food safety risk assessment and more scientific prevention and control of foodborne pathogens.

Key words: Vibrio parahaemolyticus; oligotrophic conditions; growth heterogeneity; growth model

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