UPLC-MS/MS同时测定铁皮石斛茎、叶、花中酚类组分的含量

doi:10.7506/spkx1002-6630-20200529-363

食品科学 ›› 2021, Vol. 42 ›› Issue (10): 262-268.doi: 10.7506/spkx1002-6630-20200529-363

UPLC-MS/MS同时测定铁皮石斛茎、叶、花中酚类组分的含量

黄彪，何伟，吴建鸿，王红梅，李巍

（1.福建省农业科学院农业质量标准与检测技术研究所，福建福州 350003；2.福建省农产品质量安全重点实验室，福建福州 350003；3.福建连天福生物科技有限公司，福建龙岩 366200）

出版日期:2021-05-25 发布日期:2021-06-02
基金资助:
福建省属公益类科研院所专项（2019R1022-3）；福建省农业科学院青年英才计划项目（YC2017-9）；福建省农业科学院农产品质量安全创新团队项目（STIT2017-1-12）

Simultaneous Determination of Phenolic Components in Dendrobium officinale Stems, Leaves and Flowers by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

HUANG Biao, HE Wei, WU Jianhong, WANG Hongmei, LI Wei

(1. Institute of Quality Standards and Testing Technology for Agro-products, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 2. Fujian Key Laboratory of Agro-products Qualitiy & Safety, Fuzhou 350003, China; 3. Fujian, Liantianfu Biology Technology Co. Ltd., Longyan 366200, China)

Online:2021-05-25 Published:2021-06-02

摘要/Abstract

摘要： 建立超高效液相色谱-串联质谱同时、快速分析18 种酚类物质的方法，测定比较铁皮石斛茎、叶、花部位含量差异。样品用甲醇-水溶液提取，提取液中的目标化合物经Waters T3 C18柱梯度洗脱分离，电喷雾串联三重四极杆质谱仪正、负离子扫描方式多反应监测模式检测。结果表明：以18 种酚类物质标准品作为对照，在铁皮石斛样品中共检出15 种酚类组分，分别为3,5-二羟基苯甲酸、橘皮素、绿原酸、槲皮素、芦丁、对羟基苯甲酸、咖啡酸、丁香酸、香草酸、山柰酚、对香豆酸、芥子酸、阿魏酸、3-羟基肉桂酸、水杨酸。铁皮石斛不同部位多酚组分含量为花＞叶＞茎，相对于其他酚类组分，芦丁在铁皮石斛茎、叶、花中的含量均为最高。铁皮石斛不同部位主要酚类组分表现出一定的差别：铁皮石斛花中主要含有芦丁、咖啡酸、阿魏酸和对羟基苯甲酸，铁皮石斛叶中芦丁和阿魏酸的含量相对较高，而铁皮石斛茎部主要酚类组分则为芦丁、阿魏酸与香草酸。实验结果可为铁皮石斛不同部位酚类组分的利用提供一定的科学依据。

关键词: 铁皮石斛；超高效液相色谱-串联质谱；多反应监测；酚类；测定方法

Abstract: The study aimed to compare the phenolic components in Dendrobium officinale stems, leaves and flowers. An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was established to determine the phenolic components. The samples were extracted with methanol in water, and the extract was separated on a Wates T3 C18 column by gradient elution. Then, the target compounds were analyzed by positive and negative electrospray ionization under the multiple reaction monitoring (MRM) mode. By employing 18 standard phenolic compounds as references, 15 phenolic compounds were detected in D. officinale samples, including 3,5-dihydroxybenzoic acid, tangeretin, chlorogenic acid, quercetin, rutinum, p-hydroxybenzoic acid, caffeic acid, syringate, kaempferol, vanillic acid, coumalic acid, sinapic acid, ferulic acid, 3-hydrocycinnamic acid, and salicylic acid. The contents of phenolic compounds in different parts of D. officinale were in the descending order of flowers > leaves > stems. Rutin was identified as the most dominant phenolic compound in the three parts There were differences in the major phenolic compounds in them. The major phenolic compounds in D. officinale flowers were rutin, caffeic acid, ferulic acid and p-hydroxybenzoic acid. The contents of rutin and caffeic acid in the leaves were higher than those of other phenolic compounds. The major phenolic compounds in the stems were rutinum, ferulic acid and vanillic acid. The results of this study may provide a scientific reference for the exploitation of phenolic substances in different parts of D. officinale.

Key words: Dendrobium officinale; ultra-high performance liquid chromatography-tandem mass spectrometry; multiple reaction monitoring; phenolic components; detection methods

中图分类号:

O657.72

黄彪，何伟，吴建鸿，王红梅，李巍. UPLC-MS/MS同时测定铁皮石斛茎、叶、花中酚类组分的含量[J]. 食品科学, 2021, 42(10): 262-268.

HUANG Biao, HE Wei, WU Jianhong, WANG Hongmei, LI Wei. Simultaneous Determination of Phenolic Components in Dendrobium officinale Stems, Leaves and Flowers by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2021, 42(10): 262-268.

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UPLC-MS/MS同时测定铁皮石斛茎、叶、花中酚类组分的含量

Simultaneous Determination of Phenolic Components in Dendrobium officinale Stems, Leaves and Flowers by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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