调味香料荜茇精油中清除游离基活性成分的快速筛选

doi:10.7506/spkx1002-6630-20200701-016

食品科学 ›› 2021, Vol. 42 ›› Issue (14): 226-231.doi: 10.7506/spkx1002-6630-20200701-016

调味香料荜茇精油中清除游离基活性成分的快速筛选

王婵，李荣，吴杨，王颖，汤书华，谭津，姜子涛

（1.天津商业大学生物技术与食品科学学院，天津 300134；2.天津埃文森科技有限公司，天津 300384；3.天津天狮学院食品工程学院，天津 301700）

发布日期:2021-07-27
基金资助:
天津市自然科学基金项目（16JCYBJC43300；17JCQNJC02400）

Rapid Screening of Radical Scavengers in Essential Oil from Piper longum L. as Food Seasoning

WANG Chan, LI Rong, WU Yang, WANG Ying, TANG Shuhua, TAN Jin, JIANG Zitao

(1. School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China;2. Tianjin Evans Science and Technology Co. Ltd., Tianjin 300384, China;3. School of Food Engineering, Tianjin Tianshi College, Tianjin 301700, China)

Published:2021-07-27

摘要/Abstract

摘要： 利用超快速气相色谱电子鼻（gas chromatography-based electronic nose，GC-E-nose）技术快速鉴定调味香料荜茇精油（essential oil from Piper longum L.，EOPL）的化学成分，并通过GC-E-nose结合化学法，在未经单体分离的条件下，对EOPL中的化学成分清除1,1-二苯基-2-三硝基苯肼（1,1-dipheny1-2-picrylhydrazyl，DPPH）自由基、2,2’-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐（2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate)，ABTS）阳离子自由基和羟自由基活性进行评价。结果表明，EOPL的主要成分为大根香叶烯（26.04%）、石竹烯（13.57%）、顺式-茴香脑（8.87%）和β-甜没药烯（7.32%）。EOPL各组分清除自由基的活性有很大差异，在所有精油成分中顺式-茴香脑、氧化石竹烯和右旋萜二烯对DPPH自由基、ABTS阳离子自由基和羟自由基具有最强的清除活性，清除率分别达到78.1%、87.0%和71.6%。为了验证GC-E-nose对精油成分鉴定的可行性，同时采用气相色谱-质谱联用技术对EOPL的化学成分进行分析，符合度高达89.7%。

关键词: 荜茇精油；超快速气相色谱电子鼻；气相色谱-质谱联用技术；快速筛选；清除自由基

Abstract: In the present work, the chemical components of the essential oil from Piper longum L. (EOPL) were quickly identified with an ultra-fast gas chromatography-based electronic nose (GC-E-nose). The scavenging activities of each component of EOPL against 1,1-dipheny1-2-picrylhydrazyl (DPPH) radicals, 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) cation radicals, and hydroxyl radicals were evaluated by GC-E-nose combined with chemical methods without time-consuming and laborious separation of the monomers. The results showed that the main components of EOPL identified by GC-E-nose were germacrene D (26.04%), caryophyllene (13.57%), cis-anetholel (8.87%), and β-bisabolene (7.32%). Among all identified components, cis-anethol, caryophyllene oxide, and D-limonene had the strongest scavenging capacity against DPPH radicals, ABTS cation radicals, and hydroxyl radicals, with scavenging percentages of 78.1%, 87.0%, and 71.6%, respectively. The results of GC-E-nose for essential oil components were 89.7% consisted with those of gas chromatography-mass chromatography (GC-MS), confirming the feasibility of GC-E-nose.

Key words: essential oil from Piper longum L.; ultra-fast gas chromatography-based electronic nose; gas chromatography-mass spectrometry; quick screening; radical scavenging activity

中图分类号:

TS201.3

王婵，李荣，吴杨，王颖，汤书华，谭津，姜子涛. 调味香料荜茇精油中清除游离基活性成分的快速筛选[J]. 食品科学, 2021, 42(14): 226-231.

WANG Chan, LI Rong, WU Yang, WANG Ying, TANG Shuhua, TAN Jin, JIANG Zitao. Rapid Screening of Radical Scavengers in Essential Oil from Piper longum L. as Food Seasoning[J]. FOOD SCIENCE, 2021, 42(14): 226-231.

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调味香料荜茇精油中清除游离基活性成分的快速筛选

Rapid Screening of Radical Scavengers in Essential Oil from Piper longum L. as Food Seasoning

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