RP-HPLC混标加样增量法快速测定食品中的阿斯巴甜和阿力甜

doi:10.7506/spkx1002-6630-20200729-367

食品科学 ›› 2020, Vol. 41 ›› Issue (24): 340-345.doi: 10.7506/spkx1002-6630-20200729-367

• 安全检测 • 上一篇

RP-HPLC混标加样增量法快速测定食品中的阿斯巴甜和阿力甜

高向阳，张芳

（郑州科技学院食品科学与工程学院，郑州市食品安全快速检测重点实验室，河南郑州 450064）

出版日期:2020-12-25 发布日期:2020-12-28
基金资助:
郑州市科技局新兴产业研究开发计划项目（153PXXCY186）

Rapid Determination of Aspartame and Alitame in Food by Reversed-Phase High Performance Liquid Chromatography with Mixed Standard and Sample Increment Method

GAO Xiangyang, ZHANG Fang

(Key Laboratory of Food Safety Rapid Detection in Zhengzhou, College of Food Science and Engineering, Zhengzhou University of Science and Technology, Zhengzhou 450064, China)

Online:2020-12-25 Published:2020-12-28

摘要/Abstract

摘要： 建立一种快速测定食品中阿斯巴甜和阿力甜的新方法。以饮料和酸奶为样品，用反相高效液相色谱混标加样增量法同时进行定性和定量分析。结果表明：饮料和液态乳制品中阿斯巴甜和阿力甜的检出限为0.21 mg/kg和0.020 mg/kg，回收率为90.5%～98.3%，相对标准偏差小于5%。与国标法对照测定结果，经检验无显著性差异。该法无需测定空白，无需绘制标准曲线，简便、快速、成本低，实现了标准溶液和试液同条件下的同时定性、定量分析，用于食品中的阿斯巴甜和阿力甜的快速测定，结果令人满意。

关键词: 反相高效液相色谱；混合标准溶液；加样增量法；阿斯巴甜；阿力甜；同时定性、定量

Abstract: A new rapid method for the simultaneous qualitative and quantitative analysis of aspartame and alitame in beverage and yogurt was established using reversed-phase high performance liquid chromatography with mixed standard and sample increment method. The results showed that the minimum detection limits of aspartame and alitame in beverages and liquid dairy products were 0.21 and 0.020 mg/kg, respectively. The recoveries ranged from 90.5% to 98.3%, with relative standard deviation (RSD) less than 5%. The results obtained from the proposed method showed no significant difference compared with the national standard method. This method does not need to measure blanks and draw a standard curve. It is simple, fast and low-cost, and allows the simultaneous qualitative and quantitative analysis of standard and sample solutions under the same conditions. Satisfactory results can be obtained when it is used for the rapid determination of aspartame and alitame in food.

Key words: reversed-phase high performance liquid chromatography; mixed standard solution; incremental sampling method; aspartame; alitame; simultaneous qualitative and quantitative analysis

中图分类号:

TS207.3

高向阳，张芳. RP-HPLC混标加样增量法快速测定食品中的阿斯巴甜和阿力甜[J]. 食品科学, 2020, 41(24): 340-345.

GAO Xiangyang, ZHANG Fang. Rapid Determination of Aspartame and Alitame in Food by Reversed-Phase High Performance Liquid Chromatography with Mixed Standard and Sample Increment Method[J]. FOOD SCIENCE, 2020, 41(24): 340-345.

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RP-HPLC混标加样增量法快速测定食品中的阿斯巴甜和阿力甜

Rapid Determination of Aspartame and Alitame in Food by Reversed-Phase High Performance Liquid Chromatography with Mixed Standard and Sample Increment Method

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