南极磷虾油对硫酸葡聚糖钠盐诱导溃疡性结肠炎小鼠的抗氧化作用机制

doi:10.7506/spkx1002-6630-20200831-417

食品科学 ›› 2022, Vol. 43 ›› Issue (1): 156-163.doi: 10.7506/spkx1002-6630-20200831-417

• 营养卫生 • 上一篇

南极磷虾油对硫酸葡聚糖钠盐诱导溃疡性结肠炎小鼠的抗氧化作用机制

周晓玲，相兴伟，周宇芳，郑斌，邓尚贵，廖妙飞，闻正顺

（1.浙江海洋大学食品与药学学院，浙江舟山 316022；2.浙江省海洋开发研究院，浙江舟山 316021；3.浙江工业大学食品科学与工程学院，浙江杭州 310014）

发布日期:2022-01-29
基金资助:
浙江省重点研发计划项目（2019C02076）；舟山市科技支撑“五大会战”重大科技项目（2017C12030）

Mechanism of the Antioxidant Action of Antarctic Krill Oil in Mice with Ulcerative Colitis Induced by Dextran Sulfate Sodium

ZHOU Xiaoling, XIANG Xingwei, ZHOU Yufang, ZHENG Bin, DENG Shanggui, LIAO Miaofei, WEN Zhengshun

(1. College of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, China;2. Zhejiang Marine Development Research Institute, Zhoushan 316021, China;3. College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China)

Published:2022-01-29

摘要/Abstract

摘要： 目的：探讨南极磷虾油（Antarctic krill oil，AKO）对硫酸葡聚糖钠盐（dextran sulfate sodium，DSS）诱导溃疡性结肠炎（ulcerative colitis，UC）小鼠的抗氧化作用及其机制。方法：BALB/c小鼠随机分成4 组：对照组、DSS组、低剂量AKO组（L-AKO，0.25 g/（kg mb·d））和高剂量AKO组（H-AKO，0.5 g/（kg mb·d））。通过在小鼠饮水中连续一周添加含质量分数3.5%的DSS诱导小鼠以产生UC。处死小鼠后收集其结肠组织，使用苏木精-伊红染色观察组织切片结构变化，并测定结肠组织中的谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）、超氧化物歧化酶（superoxide dismutase，SOD）的活力，以及白细胞介素-6（interleukin-6，IL-6）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、内毒素（lipopolysaccharides，LPS）、二胺氧化酶（diamine oxidase，DAO）的水平；利用实时荧光定量聚合酶链式反应（quantitative polymerase chain reaction，qPCR）、蛋白免疫印迹（Western blot）进一步测定小鼠结肠中核因子E2相关因子2（nuclear factor erythroid 2-related factor 2，Nrf2）/Keap1信号通路相关基因及关键蛋白表达水平。结果：H-AKO处理不仅可以改善DSS干预后造成的结肠组织损伤，还可以显著提高其抗氧化酶基因（GSH-Px、SOD）的表达（P＜0.05），显著降低炎症因子和肠道通透性指标（IL-6、TNF-α、LPS、DAO）的水平（P＜0.05）。qPCR结果表明相较于DSS模型组，H-AKO干预除了能提高结肠组织中Nrf2基因表达，还能提高GSH-Px、SOD和HO-1基因表达。Western blot结果显示，AKO处理使UC小鼠结肠Nrf2蛋白水平增加，Keap1蛋白水平表达降低，提示Nrf2/Keap1信号通路被激活。结论：AKO对UC小鼠的抗氧化保护作用可能是通过调节Nrf2/Keap1信号通路相关基因和蛋白表达，增强体内抗氧化酶水平的表达，从而实现改善机体氧化应激损伤、炎症反应和恢复肠道屏障结构与功能。

关键词: 南极磷虾油；溃疡性结肠炎；核因子E2相关因子2；抗氧化；炎症

Abstract: Objective: To explore the antioxidant effect and underlying mechanism of Antarctic krill oil (AKO) in mice suffering from dextran sulfate sodium (DSS)-induced ulcerative colitis (UC). Methods: BALB/c mice were randomly divided into four groups: normal, UC model, low-dose AKO (L-AKO, 0.25 g/(kg mb·d)) and high-dose (H-AKO, 0.5 g/(kg mb·d)) AKO treatments. Mice were induced to develop UC by adding 3.5% DSS to their drinking water for one week. Colon tissues were collected after the mice were euthanized to observe histological changes by hematoxylin-eosin (H&E) staining, and the expression levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), lipopolysaccharides (LPS), and diamine oxidase (DAO) in colon tissues were detected. Quantitative polymerase chain reaction (qPCR) and Western blot were used to further determine the expression of genes and key proteins related to the nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway in the colon. Results: H-AKO treatment could not only attenuate colon injury caused by DSS intervention, but also significantly increase the expression of antioxidant enzyme genes (P < 0.05), and reduce the levels of inflammatory factors and intestinal permeability indicators (IL-6, TNF-α, LPS and DAO) (P < 0.05) in colon tissues. qPCR results showed that H-AKO increased the gene expression of Nrf2 as well as GSH-Px, SOD and HO-1. Western blot results showed that AKO treatment activated the Nrf2 signaling pathway by increasing Nrf2 protein and decreasing Keap1 protein expression. Conclusion: AKO can exert antioxidant protection in UC mice through activating the expression of genes and proteins related to the Nrf2 signaling pathway, enhancing the expression of antioxidant enzymes, thereby reducing oxidative stress injury, suppressing inflammatory response, and restoring intestinal barrier structure and function.

Key words: Antarctic krill oil; ulcerative colitis; nuclear factor erythroid 2-related factor 2; antioxidant; inflammation

中图分类号:

TS254.9

周晓玲，相兴伟，周宇芳，郑斌，邓尚贵，廖妙飞，闻正顺. 南极磷虾油对硫酸葡聚糖钠盐诱导溃疡性结肠炎小鼠的抗氧化作用机制[J]. 食品科学, 2022, 43(1): 156-163.

ZHOU Xiaoling, XIANG Xingwei, ZHOU Yufang, ZHENG Bin, DENG Shanggui, LIAO Miaofei, WEN Zhengshun. Mechanism of the Antioxidant Action of Antarctic Krill Oil in Mice with Ulcerative Colitis Induced by Dextran Sulfate Sodium[J]. FOOD SCIENCE, 2022, 43(1): 156-163.

[1]	高瑾梁宏闪赵靖昀代亚磊万楚筠周彬. 玉米醇溶蛋白/多酚相互作用及复合物制备与表征[J]. , 0, (): 0-0.
[2]	周晓玲相兴伟周宇芳郑斌邓尚贵廖妙飞闻正顺. 南极磷虾油对硫酸葡聚糖钠盐诱导溃疡性结肠炎小鼠的抗氧化作用机制[J]. , 0, (): 0-0.
[3]	张玲董婧媛陈永德李茜吴涛刘锐隋文杰张民. 海参废渣皂苷通过NOD样受体信号通路抵抗环磷酰胺诱导小鼠的免疫抑制[J]. 食品科学, 0, (): 0-0.
[4]	刘洋肖宇马爱进桑亚新孙纪录. 超微粉碎和高压均质联合处理对几丁质理化性质及微观结构的影响[J]. 食品科学, 0, (): 0-0.
[5]	孙慧慧杨国淞程伊梦李昭悦赵玲曹荣刘淇. 海洋寡糖的生物活性研究进展[J]. 食品科学, 0, (): 0-0.
[6]	李学鹏王媛媛王金厢励建荣李婷婷郭晓华. 蒸煮方式对鳕鱼头汤呈味特性的影响[J]. , 0, (): 0-0.
[7]	王红丽王锡昌施文正周纷王玥科. 水产品贮运过程中货架期预测的研究进展[J]. , 0, (): 0-0.
[8]	王媛媛，李学鹏，王金厢，励建荣，李婷婷，郭晓华. 蒸煮方式对鳕鱼头汤呈味特性的影响[J]. 食品科学, 2021, 42(15): 58-65.
[9]	王舒雅赵靖昀高瑾梁宏闪李述刚周彬. 鱼皮明胶和多酚组装行为与相互作用[J]. , 0, (): 0-0.
[10]	张晓頔，戴志远. 鱼副产物蛋白水解物生物活性及应用研究进展[J]. 食品科学, 2021, 42(13): 335-343.
[11]	张晓頔戴志远. 鱼副产物蛋白水解物生物活性及应用研究进展[J]. , 0, (): 0-0.
[12]	陈菁，杜振兴，陈建平，贾学静，刘晓菲，李瑞，钟赛意. 虾头类肝素的制备、理化性质及抗凝血活性评价[J]. 食品科学, 2021, 42(11): 71-77.
[13]	杜杰，刘廷薇，马良，王洪霞，戴宏杰，余永，朱翰昆，张宇昊. 微波-快速冻融耦合鱼皮明胶理化性质分析[J]. 食品科学, 2021, 42(11): 108-115.
[14]	杜杰刘廷薇马良王洪霞戴宏杰余永朱瀚昆张宇昊. 微波-快速冻融耦合鱼皮明胶理化性质分析[J]. 食品科学, 0, (): 0-0.
[15]	陈菁杜振兴陈建平贾学静钟赛意. 虾头类肝素的制备、理化性质及抗凝血活性评价[J]. 食品科学, 0, (): 0-0.

南极磷虾油对硫酸葡聚糖钠盐诱导溃疡性结肠炎小鼠的抗氧化作用机制

Mechanism of the Antioxidant Action of Antarctic Krill Oil in Mice with Ulcerative Colitis Induced by Dextran Sulfate Sodium

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价