促人胎结肠上皮细胞增殖的婴儿源双歧杆菌的分离筛选及生物学性质

doi:10.7506/spkx1002-6630-20200914-179

食品科学 ›› 2021, Vol. 42 ›› Issue (18): 86-94.doi: 10.7506/spkx1002-6630-20200914-179

促人胎结肠上皮细胞增殖的婴儿源双歧杆菌的分离筛选及生物学性质

关嘉琦，梁胜男，陈庆学，赵丽娜，解庆刚，霍贵成，李柏良

（1.东北农业大学食品学院，黑龙江哈尔滨 150030；2.黑龙江飞鹤乳业有限公司，黑龙江齐齐哈尔 164800）

发布日期:2021-09-29
基金资助:
“十三五”国家重点研发计划重点专项（2017YFD0400303）

Screening for and Biological Characterization of Bifidobacterium infantis Capable of Promoting Proliferation of Human Fetal Colon Epithelial Cells

GUAN Jiaqi, LIANG Shengnan, CHEN Qingxue, ZHAO Lina, XIE Qinggang, HUO Guicheng, LI Bailiang

(1. College of Food Science, Northeast Agricultural University, Harbin 150030, China;2. Heilongjiang Feihe Dairy Co. Ltd., Qiqihar 164800, China)

Published:2021-09-29

摘要/Abstract

摘要： 以分离得到的15 株婴儿源双歧杆菌作为实验菌株，研究其对人胎结肠上皮细胞（CCD841 CoN）的增殖促进效果，以期筛选出一株具有较强促增殖能力的双歧杆菌，并对其形态学特征、生长特性、环境耐受性、黏附性及安全性等生物学性质进行评价，确定最佳促增殖条件。通过CCK-8法分别分析各实验菌株活菌及灭活菌菌悬液、发酵上清液及裂解液对CCD841 CoN细胞的增殖活性百分比，主成分分析结果表明Bifidobacterium ongum K2-21-4综合促增殖性能最佳。对CCD841 CoN细胞的黏附性实验也显示，K2-21-4的黏附率仅次于商业菌株Lactobacillus animalis BB12，可达6.6%，然而，B. longum K2-21-4对酸、胆盐的耐受性较差。除对氨基糖苷类、喹诺酮类抗生素具有抗性外，对青霉素、大环内酯类具有一定程度敏感，对阿莫西林、万古霉素、四环素、利福平、氯霉素均敏感，无溶血活性。107 CFU/mL长双歧杆菌K2-21-4活菌悬液与细胞共作24 h的细胞增殖促进作用最佳。综上B. longum K2-21-4具有较高的促进CCD841 CoN细胞增殖能力，具有一定的应用潜力。

关键词: 双歧杆菌；CCD841 CoN细胞；细胞增殖；生物学特性；婴儿粪便

Abstract: Fifteen strains of Bifidobacterium infantis were screened for their promoting effects on the proliferation of human fetal colon epithelial cells (CCD841 CoN) in this study. The biological properties of the selected strains such as morphological characteristics, growth characteristics, environmental tolerance, adhesion properties and safety were evaluated, and the optimal conditions for promoting cell proliferation were also determined. The effect of live and inactivated cell suspensions, culture supernatants, and lysates of each strain on the proliferation activity of CCD841 CoN cells was evaluated by CCK-8 method. The results of principal component analysis (PCA) showed that Bifidobacterium longum K2-21-4 had the best overall cell proliferation-promoting effect. The adhesion rate of K2-21-4 to CCD841 CoN cells was 6.6%, only slightly lower than that of the commercial strain Lactobacillus animalis BB12. However, the tolerance of K2-21-4 to acid and bile salt was poor. K2-21-4 had resistance to aminoglycosides and quinolones but was to a certain extent sensitive to penicillin and macrolide, and highly sensitive to amoxicillin, vancomycin, tetracycline, rifampicin and chloramphenicol. It had no hemolytic activity. The live cell suspension of B. longum K2-21-4 at 107 CFU/mL had the best effect on promoting cell proliferation when co-cultured for 24 h. To sum up, B. longum K2-21-4 can be potentially applied to promote the proliferation of CCD841 CoN cells.

Key words: Bifidobacterium; CCD841 CoN cells; cell proliferation; biological characteristics; baby feces

中图分类号:

Q939.99

关嘉琦，梁胜男，陈庆学，赵丽娜，解庆刚，霍贵成，李柏良. 促人胎结肠上皮细胞增殖的婴儿源双歧杆菌的分离筛选及生物学性质[J]. 食品科学, 2021, 42(18): 86-94.

GUAN Jiaqi, LIANG Shengnan, CHEN Qingxue, ZHAO Lina, XIE Qinggang, HUO Guicheng, LI Bailiang. Screening for and Biological Characterization of Bifidobacterium infantis Capable of Promoting Proliferation of Human Fetal Colon Epithelial Cells[J]. FOOD SCIENCE, 2021, 42(18): 86-94.

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促人胎结肠上皮细胞增殖的婴儿源双歧杆菌的分离筛选及生物学性质

Screening for and Biological Characterization of Bifidobacterium infantis Capable of Promoting Proliferation of Human Fetal Colon Epithelial Cells

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