关键词: 鼠伤寒沙门氏菌；外泌体；蛋白质组学

Abstract: In our present study, the human intestinal epithelial cell line Henle-407 was used as a host model. Label-free quantitative proteomics was used to explore the change of host proteome in exosomes secreted by host cells infected with S. typhimurium. A total of 2 490 proteins were quantitated, including 321 differentially expressed proteins (DEPs) in exosomes from S. typhimurium-infected and uninfected Henle-407 cells. Seven DEPs were common to both, three of which were up-regulated and the rest were down-regulated. The remaining 314 DEPs were not shared between them, nine of which were specifically present in the exosomes from S. typhimurium-infected Henle-407 cells, while 305 were missing. Gene Ontology (GO) analysis indicated that the DEPs were mainly distributed in organelles and membrane, involved in metabolism and biological regulation processes, and related to molecular functions such as binding, catalytic activity and transport. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the DEPs were associated with 17 specific KEGG pathways. By comparing the host proteomics data of exosomes from S. typhimurium-infected and uninfected Henle-407 cells, it was found that infected S. typhimurium led to a sharp reduction in the amount of host cell-derived proteins in exosomes in addition to altering the expression of a few shared DEPs. Considering that exosome have been reported as double-layered membrane vesicles with a particle size of 30–150 nm, we speculated that in a limited space, Salmonella infection may inhibit the transport of host cell proteins to exosomes by unknown mechanisms. The specific proteins and lipopolysaccharides of Salmonella enter exosomes and promote the infection and spread of Salmonella in host cells by changing the susceptibility of neighboring cells to Salmonella.

Key words: Salmonella typhimurium; exosome; proteomics