鸡蛋中氟苯尼考质控样品的研制及稳定性评价

doi:10.7506/spkx1002-6630-20210629-330

食品科学 ›› 2022, Vol. 43 ›› Issue (10): 315-321.doi: 10.7506/spkx1002-6630-20210629-330

鸡蛋中氟苯尼考质控样品的研制及稳定性评价

蔡若纯，邓皇翼，刘耀慧，刘鹏展，罗志浩，刘海虹，雷毅

（广东省食品检验所，广东广州 510435）

出版日期:2022-05-25 发布日期:2022-05-27
基金资助:
广东省市场监督管理局科技项目（2021ZS02）；广东省食品检验所2019年科技创新项目（2019JS02）

Preparation and Stability of Quality Control Samples for the Determination of Florfenicol in Eggs

CAI Ruochun, DENG Huangyi, LIU Yaohui, LIU Pengzhan, LUO Zhihao, LIU Haihong, LEI Yi

(Guangdong Institute of Food Inspection, Guangzhou 510435, China)

Online:2022-05-25 Published:2022-05-27

摘要/Abstract

摘要： 采用空白鸡蛋基质中添加不同含量氟苯尼考的方式自主研制质控样品，按照CNAS-GL003:2018《能力验证样品均匀性和稳定性评价指南》，采用GB/T 22338—2008《动物源性食品中氯霉素类药物残留量测定》液相色谱-串联质谱法为参比方法对质控样品的均匀性和稳定性进行评价，同时对影响质控样品稳定性的因素进行研究，并通过6 家实验室间联合定值的方式确定质控样品的参考值，结果表明自主研制的非冻干（液态）和冻干（固态）2 种状态质控样品均匀性良好，不同样品状态、不同贮存温度对质控样品稳定性有影响，冻干质控样品在－18 ℃条件下贮存12 个月稳定性良好，3 个添加量的冻干质控样品最终定值结果为0.23、0.39 μg/kg和1.92 μg/kg，可推广应用于快速检测领域和实验室日常检测的质量控制。

关键词: 氟苯尼考；质控样品；均匀性；稳定性；定值

Abstract: Quality control samples were independently developed by adding different concentrations of florfenicol to blank eggs. According to CNAS-GL003:2018 Guidelines on Evaluating the Homogeneity and Stability of Samples used for proficiency Testing, their homogeneity and stability were evaluated by using liquid chromatography-mass spectrometry (LC-MS) specified in GB/T 22338-2008 as a reference method. At the same time, the factors affecting the stability of the quality control samples were studied, and reference values for the quality control samples were determined jointly by six laboratories. The results showed that both the non-freeze-dried (liquid) and freeze-dried (solid) quality control samples had good homogeneity and stability. Sample state and storage temperature had an impact on the stability of the quality control samples. The freeze-dried quality control samples were stable at ?18 ℃ for 12 months. The certified values for the freeze-dried quality control samples at three concentration levels were 0.23, 0.39 and 1.92 μg/kg, and these quality control samples could be applied for process quality control in the fields of rapid testing and conventional laboratory detection.

Key words: florfenicol; quality control samples; homogeneity; stability; characterization

中图分类号:

O657

蔡若纯，邓皇翼，刘耀慧，刘鹏展，罗志浩，刘海虹，雷毅. 鸡蛋中氟苯尼考质控样品的研制及稳定性评价[J]. 食品科学, 2022, 43(10): 315-321.

CAI Ruochun, DENG Huangyi, LIU Yaohui, LIU Pengzhan, LUO Zhihao, LIU Haihong, LEI Yi. Preparation and Stability of Quality Control Samples for the Determination of Florfenicol in Eggs[J]. FOOD SCIENCE, 2022, 43(10): 315-321.

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鸡蛋中氟苯尼考质控样品的研制及稳定性评价

Preparation and Stability of Quality Control Samples for the Determination of Florfenicol in Eggs

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