食品科学 ›› 2022, Vol. 43 ›› Issue (14): 183-192.doi: 10.7506/spkx1002-6630-20210701-003

• 生物工程 • 上一篇    

基于孢粉素微胶囊的共包埋、保护及递送益生菌和乳糖酶的体系

邓紫玙,丁一,侯欣尧,李斌,梁宏闪   

  1. (华中农业大学食品科技学院,环境食品学教育部重点实验室,湖北 武汉 430070)
  • 发布日期:2022-07-28
  • 基金资助:
    湖北省本科生科技创新专项(201510318255);国家自然科学基金青年科学基金项目(31801586)

Sporopollenin Microcapsule-based System for Co-encapsulation, Protection and Delivery of Probiotics and β-Galactosidase

DENG Ziyu, DING Yi, HOU Xinyao, LI Bin, LIANG Hongshan   

  1. (Key Laboratory of Environment Correlative Dietology, Ministry of Education, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)
  • Published:2022-07-28

摘要: 研究旨在开发基于天然植物微胶囊的核壳结构载体,作为益生菌和乳糖酶的递送及保护体系。该核壳结构的孢粉素外壁胶囊(sporopollenin exine capsules,SECs)为核,海藻酸钙(Ca-alginate,Ca-Alg)/羧甲基茯苓多糖(carboxymethylpachymaran,CMP)凝胶为壳,对益生菌的负载量高达9.63×109 CFU/g,乳糖酶活力保留率高达80.72%。在Ca-Alg/CMP壳中,CMP引入可以改变凝胶壳层对水的结合能力,从而改变其溶胀行为和微观结构,借此影响体系中益生菌和乳糖酶的释放行为。结果表明,引入CMP可使Ca-Alg/CMP凝胶包裹的负载益生菌及乳糖酶的SECs在胃肠道中表现出更加优异的稳定性:在模拟消化600 min后,活菌数超过107 CFU/mL,酶活力保留率约为62%。相比于纯菌液和纯酶液,该体系可减少冻干和贮存处理后菌数和酶活力损失率(P<0.05)。最后,利用甲基纤维素对核壳结构进行二次包埋,可提高体系中益生菌和乳糖酶的热稳定性(P<0.05)。

关键词: 孢粉素外壁胶囊;核壳结构;羧甲基茯苓多糖;益生菌;乳糖酶;稳定性

Abstract: This study aimed to develop a core-shell carrier based on natural plant microcapsules for use as a delivery system for Lactobacillus plantarum and β-galactosidase (β-Gal). Sporopollenin exine capsules (SECs) were used as the core, and calcium-alginate (Ca-Alg)/carboxymethylpachymaran (CMP) as the shell. The probiotics loading capacity and the residual activity of β-Gal were 9.63 × 109 CFU/g and 80.72%, respectively. The introduction of CMP could affect the swelling behavior and microstructure of the shell and, consequently, the release behavior of probiotics and enzymes by changing its water-binding capacity. The introduction of CMP improved the gastrointestinal stability of Ca-Alg/CMP coated SECs loaded with L. plantarum and β-Gal. After 600 min of culture under simulated gastrointestinal conditions, the number of viable cells exceeded 107 CFU/mL, and the residual activity of β-Gal was around 62%. Compared with the control group (pure L. plantarum and β-Gal), the system could significantly reduce the loss rates of the probiotic cell count and β-Gal activity after lyophilization and during storage (P < 0.05). Finally, using methylcellulose (MC) to coat the core-shell structure could significantly improve the thermal stability of L. plantarum and β-Gal (P < 0.05).

Key words: sporopollenin exine capsules; core-shell structure; carboxymethylpachymaran; probiotic; enzyme; stability

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