食品科学 ›› 2022, Vol. 43 ›› Issue (16): 177-185.doi: 10.7506/spkx1002-6630-20210830-389

• 生物工程 • 上一篇    下一篇

六堡茶渥堆过程中可培养微生物的分离与鉴定

黄紫衡,陈欢,黄丽,夏宁,滕建文,韦保耀,陈瑜,余炼   

  1. (1.广西大学轻工与食品工程学院,广西 南宁 530000;2.贵州省兴义市敬南镇人民政府,贵州 兴义 562402)
  • 出版日期:2022-08-25 发布日期:2022-08-31
  • 基金资助:
    国家自然科学基金地区科学基金项目(32160571)

Isolation and Identification of Culturable Microbes during the Fermentation of Liupao Tea

HUANG Ziheng, CHEN Huan, HUANG Li, XIA Ning, TENG Jianwen, WEI Baoyao, CHEN Yu, YU Lian   

  1. (1. Institute of Light Industry and Food Engineernig, Guangxi University, Nanning 530000, China; 2. Jingnan Town People’s Government of Xingyi City, Guizhou Province, Xingyi 562402, China)
  • Online:2022-08-25 Published:2022-08-31

摘要: 采用传统微生物分离和分子鉴定技术对六堡茶渥堆过程的样品中微生物多样性进行研究。从不同渥堆阶段的样品中分离出细菌13 个属、27 个种,放线菌7 个属、8 个种,真菌11 个属、23 个种,其中黑曲霉、塔宾曲霉、Blastobotrys adeninivorans和芽孢杆菌属在整个渥堆过程中都检出。本研究所有分离鉴定得到的细菌22 种、放线菌7 种和真菌9 种均为六堡茶中首次发现与报道。本研究有利于了解六堡茶渥堆工艺的微生物作用,并对六堡茶的纯种发酵和微生物安全性分析提供理论依据。

关键词: 六堡茶;渥堆;微生物;分离;鉴定

Abstract: The microbial community structure in Liupao tea at different stages of pile fermentation was studied by traditional microbial isolation and molecular identification. A total of 27 bacteria belonging to 13 genus, 8 actinomycetes species belonging to 7 genus, and 23 fungal species belonging to 11 genera were identified. Aspergillus niger, Aspergillus tubingensis, Blastobotrys adeninivorans and Bacillus spp. were found at all stages of the fermentation process. Totally 22 species of bacteria, 7 species of actinomycetes and 9 species of fungi were reported first in Liupao tea. These findings are helpful to understand the microbial role in the pile fermentation of Liupao tea, and provide a theoretical basis for the pure culture fermentation and microbial safety analysis of Liupao tea.

Key words: Liupao tea; pile fermentation; microorganism; isolation; identification

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