食品科学 ›› 2022, Vol. 43 ›› Issue (18): 176-183.doi: 10.7506/spkx1002-6630-20210913-152

• 生物工程 • 上一篇    

人参、大蒜混合发酵过程中微生物多样性、成分及抗氧化能力分析

葛长锌,李雅倩,董微巍,周鑫,徐孟休,李官浩,朴春香   

  1. (1.延边大学农学院,吉林 延吉 133000;2.青岛大学化学化工学院,山东 青岛 266000;3.金乡蒜通天下仓储有限公司,山东 金乡 272202)
  • 发布日期:2022-09-28
  • 基金资助:
    吉林省重大科技专项(20200504002YY)

Analysis of Microbial Diversity, Chemical Composition and Antioxidant Capacity during Co-fermentation of Ginseng and Garlic

GE Changxin, LI Yaqian, DONG Weiwei, ZHOU Xin, XU Mengxiu, LI Guanhao, PIAO Chunxiang   

  1. (1. Agricultural College of Yanbian University, Yanji 133000, China; 2. College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266000, China; 3. Jinxiang Suantongtianxia Storage Co. Ltd., Jinxiang 272202, China)
  • Published:2022-09-28

摘要: 探究人参与大蒜混合发酵对黑参微生物多样性、成分以及生理功能的影响。以新鲜大蒜和人参为原料,采用液态发酵法自制黑蒜、黑参和参蒜,对其发酵过程中微生物多样性、主要成分以及抗氧化性进行分析。结果表明:参蒜产品的优势菌门为厚壁菌门,优势菌属为uncultured_bacterium_f_Muribaculaceae、乳杆菌属等;根据京都基因与基因组百科全书进行代谢途径预测,参蒜与大蒜和人参的代谢途径无明显差异,主要为全局及概要图、碳水化合物代谢和氨基酸代谢;参蒜的还原糖含量和总酚含量显著提高,总酸含量下降,稀有人参皂苷转化率显著提高,潜在有害物质5-羟甲基糠醛含量显著下降;参蒜具有良好的自由基清除能力。以上研究为参蒜产品的开发、促进黑参产业的发展提供理论依据。

关键词: 大蒜;人参;液态发酵;微生物多样性;成分分析;抗氧化能力

Abstract: In order to explore whether co-fermentation of garlic and ginseng could affect the microbial diversity, chemical composition and physiological function of black ginseng, fresh garlic and ginseng were used as raw materials to make black garlic, black ginseng and ginseng garlic by liquid-state fermentation. During the fermentation process, their microbial diversity, major components and antioxidant properties were analyzed. The results showed that the dominant bacterial phylum in ginseng garlic was Firmicutes, and the dominant bacterial genera included uncultured_bacterium_f_Muribaculaceae and Lactobacillus. The Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that there were no obvious differences between the metabolic pathways of ginseng garlic, black garlic and black ginseng, mainly including Global and overview maps, carbohydrate metabolism and amino acid metabolism. The contents of reducing sugars and total phenols in ginseng garlic were significantly increased, the total acid content was decreased, the conversion rate of rare ginsenosides was significantly increased, and the content of the potential harmful substance 5-hydroxymethylfurfural (5-HMF) was significantly reduced. Moreover, ginseng garlic had good free radical scavenging capacity. The above findings provide a theoretical basis for the development of ginseng garlic products and for promoting the development of the black ginseng industry.

Key words: garlic; ginseng; liquid-state fermentation; microbial diversity; chemical composition analysis; antioxidant activity

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