基于UPLC-MS/MS的3 个李品种果实初生代谢物分析

doi:10.7506/spkx1002-6630-20211009-085

食品科学 ›› 2022, Vol. 43 ›› Issue (16): 226-234.doi: 10.7506/spkx1002-6630-20211009-085

基于UPLC-MS/MS的3 个李品种果实初生代谢物分析

张琴，黄世安，林欣，罗登灿，彭俊森，朱守亮，董晓庆

（1.贵州大学农学院，贵州省果树工程技术研究中心，贵州贵阳 550025；2.贵州省果树蔬菜工作站，贵州贵阳 550025）

出版日期:2022-08-25 发布日期:2022-08-31
基金资助:
国家自然科学基金面上项目（31960620）

Analysis of Primary Metabolites in Three Plum Varieties Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHANG Qin, HUANG Shi’an, LIN Xin, LUO Dengcan, PENG Junsen, ZHU Shouliang, DONG Xiaoqing

(1. Guizhou Engineering Research Center for Fruit Crops, College of Agricultural, Guizhou University, Guiyang 550025, China; 2. Guizhou Workstation for Fruits and Vegetables, Guiyang 550025, China)

Online:2022-08-25 Published:2022-08-31

摘要/Abstract

摘要： 以蜂糖李、空心李和脆红李果实为试材，采用超高效液相色谱-串联质谱技术对其初生代谢物进行定性和定量分析；并通过主成分分析、正交偏最小二乘判别分析和样本相关性分析等方法，比较各品种之间的差异代谢物，及通过京都基因与基因组百科全书富集分析，解析代谢通路之间的差异。结果表明：3 个李品种果实共检测到12 类307 种代谢物，主要包括氨基酸及其衍生物、核苷酸及其衍生物、有机酸、糖类、醇类、游离脂肪酸等代谢物。蜂糖李与脆红李之间的差异代谢物有70 种，其中43 种物质相对含量上调，27 种下调，分别占61.43%和38.57%；差异显著的代谢途径有5 条，分别是代谢通路、氨基酰-tRNA生物合成通路、缬氨酸、亮氨酸和异亮氨酸的生物合成通路、硫代葡萄糖苷的生物合成通路和赖氨酸降解通路。蜂糖李与空心李之间的差异代谢物有96 种，其中84 种物质上调和12 种物质下调，分别占87.50%和12.50%；差异显著的代谢途径有1 条为色氨酸代谢通路。空心李与脆红李之间共检测到75 种差异代谢物，其中19 种上调，56 种下调，分别占比为25.33%和74.67%；差异显著代谢途径有2 条分别是嘌呤代谢通路和硫代葡萄糖苷生物合成通路。

关键词: 李；果实；初生代谢物；超高效液相色谱-串联质谱；差异代谢物

Abstract: The primary metabolites in Fengtang, Kongxin and Cuihong plum fruits were qualitatively and quantitatively analyzed by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The differential metabolites among varieties were identified by principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), and the differential metabolic pathways were analyzed through KEGG (Kyoto Encyclopedia of Genes and Genomes) enrichment analysis. The results showed that a total of 307 metabolites belonging to 12 categories were detected, including amino acids and derivatives, nucleotides and derivatives, organic acids, sugars, alcohols, and free fatty acids. There were 70 differential metabolites between Fengtang and Cuihong plum, among which, 43 (61.43%) were up-regulated and 27 (38.57%) were down-regulated. Five significantly differential metabolic pathways were recognized, including metabolic pathways, aminoacyl-tRNA biosynthesis, valine, leucine and isoleucine degradation, glucosinolate biosynthesis and lysine degradation. In total, 96 differential metabolites were found between Fengtang and Kongxin plum, of which, 84 (87.50%) were up-regulated and 12 (12.50%) were down-regulated, and only one significantly differential metabolic pathway was identified, namely tryptophan metabolism. A total of 75 differential metabolites were found between Kongxin and Cuihong plum, of which, 19 (25.33%) were up-regulated and 56 (74.67%) were down-regulated, and two significantly differential metabolic pathways were identified, including purine metabolism and glucosinolate biosynthesis.

Key words: plum; fruits; primary metabolites; ultra-high performance liquid chromatography-tandem mass spectrometry; differential metabolites

中图分类号:

TS255.3

张琴，黄世安，林欣，罗登灿，彭俊森，朱守亮，董晓庆. 基于UPLC-MS/MS的3 个李品种果实初生代谢物分析[J]. 食品科学, 2022, 43(16): 226-234.

ZHANG Qin, HUANG Shi’an, LIN Xin, LUO Dengcan, PENG Junsen, ZHU Shouliang, DONG Xiaoqing. Analysis of Primary Metabolites in Three Plum Varieties Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. FOOD SCIENCE, 2022, 43(16): 226-234.

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基于UPLC-MS/MS的3 个李品种果实初生代谢物分析

Analysis of Primary Metabolites in Three Plum Varieties Using Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry

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