食品科学 ›› 2023, Vol. 44 ›› Issue (4): 247-255.doi: 10.7506/spkx1002-6630-20220420-257

• 成分分析 • 上一篇    下一篇

基于顶空气相离子迁移色谱对鸭肉腥味特征物质的鉴定

赵改名,王森,祝超智,孙灵霞,王壮壮,王兴辉,谢伟   

  1. (1.河南农业大学食品科学技术学院,河南 郑州 450002;2.馆陶六和食品有限公司,河北 邯郸 057750)
  • 发布日期:2023-03-01
  • 基金资助:
    河南省重大科技专项(161100110800)

Identification of Characteristic Odorants in Duck Meat by Headspace-Gas Chromatography-Ion Mobility Spectrometry

ZHAO Gaiming, WANG Sen, ZHU Chaozhi, SUN Lingxia, WANG Zhuangzhuang, WANG Xinghui, XIE Wei   

  1. (1. College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; 2. Guantao Liuhe Food Co. Ltd., Handan 057750, China)
  • Published:2023-03-01

摘要: 为探究鸭肉在60 ℃和90 ℃两种温度煮制后的腥味特征物质,以鸭胸、鸭腿及鸭锁骨为研究对象,基于顶空气相色谱-离子迁移谱(headspace-gas chromatography-ion mobility spectrometry,HS-GC-IMS)技术结合嗅闻评价、电子鼻测定及风味物质评价分析确定鸭肉腥味特征物质。结果表明,在两种温度处理下,90 ℃处理的各部位鸭肉腥味重于60 ℃,且不同处理组鸭肉样品的腥味特征具有一定差异,电子鼻分析结果也验证了不同处理组鸭肉样品气味存在一定差异。同时基于HS-GC-IMS技术共鉴定出61 种挥发性物质,鉴定发现不同处理组鸭肉样本中挥发性物质种类基本一致,而相对含量存在部分差异。结合嗅闻评价结果与风味物质的相对气味活性值共筛选出12 种腥味特征物质,分别为壬醛、辛醛、庚醛、癸醛、戊醛、己醛、(E)-2-辛烯醛、(E)-2-庚烯醛、2-甲基丁醛、3-甲基丁醛、2-庚酮和1-辛烯-3-醇,这些物质之间发生的交互作用对鸭肉腥味形成具有重要贡献,并且因种类和含量的不同使得两种温度处理的鸭肉样品各具特征腥味。

关键词: 鸭肉;嗅闻评价;电子鼻;顶空气相色谱-离子迁移谱;相对气味活性值;腥味特征物质

Abstract: The characteristic odorants in duck breast, leg and clavicle meat cooked at 60 and 90 ℃ were analyzed by headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) and were identified by sensory evaluation, electronic nose measurement and flavor substance evaluation. The results showed that duck breast, leg and clavicle meat cooked at 90 ℃ had a stronger odor than at 60 ℃, and there were differences in the odor characteristics of different groups of duck meat samples, which was also confirmed by electronic nose analysis. A total of 61 volatile flavor compounds were identified by HS-GC-IMS. The types of volatile substances in different groups of duck meat samples were basically the same, but there were differences in their relative contents. Based on the sensory evaluation results and the relative odor activity values (ROAV) of flavor substances, a total of 12 characteristic odorants were identified, including nonanal, octanal, heptanal, decanal, pentanal, hexanal, (E)-2-octenal, (E)-2-heptenal, 2-methyl-butanal, 3-methyl-butanal, 2-heptanone and 1-octene-3-ol. The interaction between these substances contributed significantly to the formation of the odor of duck meat. Due to the differences in the types and contents of odorants, duck meat samples cooked at 60 and 90 ℃ had distinct odor characteristics.

Key words: duck meat; sensory odor evaluation; electronic nose; headspace-gas chromatography-ion mobility spectrometry; relative odor activity value; characteristic odorants

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