食品科学 ›› 2023, Vol. 44 ›› Issue (6): 327-335.doi: 10.7506/spkx1002-6630-20220514-188

• 成分分析 • 上一篇    下一篇

GC-MS结合GC-O、电子鼻评价不同预处理方式对沙枣风味的影响

党昕,刘军,姚凌云,艾合买提江·艾海提,冯涛   

  1. (1.新疆大学生命科学与技术学院,新疆 乌鲁木齐 830046;2.上海应用技术大学香料香精技术与工程学院,上海 201418)
  • 出版日期:2023-03-27 发布日期:2023-03-27
  • 基金资助:
    自治区公益性科研院所基本科研业务经费资助项目(KY2021026)

Effects of Different Pretreatment Methods on the Flavor of Elaeagnus angustifolia Fruit Evaluated by Gas Chromatography-Mass Spectrometry, Gas Chromatography-Olfactometry and Electronic Nose

DANG Xin, LIU Jun, YAO Lingyun, Aihamatijiang·Aiheti, FENG Tao   

  1. (1. College of Life Science and Technology, Xinjiang University, ürümqi 830046, China;2. School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China)
  • Online:2023-03-27 Published:2023-03-27

摘要: 采用顶空固相微萃取结合气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)、气相色谱-嗅闻(gas chromatography-olfactometry,GC-O)及电子鼻方法,探究原果、烘焙、干燥及蒸、煮共5 种不同预处理方式对新疆大果沙枣挥发性风味的影响。结果表明:通过GC-MS从5 组不同处理后的沙枣中检测出69 种化合物,通过GC-O分析结合香气活力值进一步分析,确定沙枣的特征香气成分有14 种挥发性物质,发现(E,E)-2,4-癸二烯醛、壬醛、(E,E)-2,4-壬二烯醛、β-紫罗兰酮等是对沙枣具有关键香气贡献最大的物质。经过不同预处理后,样品的多个感官属性的香气强度存在显著差异,电子鼻可以有效区分各样品组之间的香气强度,通过偏最小二乘回归法进一步验证、解释不同处理下的沙枣中的特征香气强度与感官属性之间的相关性。综合分析可知,烘焙处理后的沙枣感官品质相对较好。

关键词: 预处理;沙枣;气相色谱-质谱联用法;电子鼻;偏最小二乘回归分析

Abstract: Headspace solid phase micro-extraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O) and an electronic nose were used to investigate the effects of five pretreatment methods including raw fruit, baking, drying, steaming and boiling on the volatile flavor profile of Elaeagnus angustifolia fruit grown in Xinjiang. The results showed that by GC-MS analysis, a total of 69 compounds were detected in the five samples. Based on the results of GC-O combined with odor activity value (OAV), 14 volatile compounds were identified as characteristic aroma components. It was found that (E,E)-2,4-decadienal, nonal, (E,E)-2,4-nonadienal, and β-ionone contributed most to the aroma of E. angustifolia fruit. After different pretreatments, there were significant differences in the aroma intensity for multiple sensory attributes among the samples. The electronic nose could effectively distinguish the aroma intensity of these samples. Partial least squares regression (PLSR) was used to analyze and explain the correlation between characteristic aroma intensity and sensory attributes. Overall, the sensory quality of baked E. angustifolia fruit was relatively better than that of the other samples.

Key words: pretreatment methods; Elaeagnus angustifolia; gas chromatography-mass spectrometry; electronic nose; partial least squares regression

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