食品科学 ›› 2023, Vol. 44 ›› Issue (16): 50-60.doi: 10.7506/spkx1002-6630-20221117-206

• 食品化学 • 上一篇    下一篇

酸诱导酪蛋白胶束-海藻酸钠乳液凝胶性质及其对原花青素的负载性能

乔蕾蕾,杨敏,秦娟娟,廖海周,季伟,李茜   

  1. (甘肃农业大学理学院,甘肃 兰州 730070)
  • 出版日期:2023-08-25 发布日期:2023-09-01
  • 基金资助:
    国家自然科学基金地区科学基金项目(32060549);甘肃省自然科学基金项目(22JR5RA861); 甘肃农业大学伏羲杰出人才培育计划项目(Gaufx-02J02);甘肃农业大学盛彤笙科技创新基金项目(GSAU-STS-2018-18)

Properties and Proanthocyanidin-Loading Capacity of Acid-Induced Micellar Casein-Sodium Alginate Emulsion Gels

QIAO Leilei, YANG Min, QIN Juanjuan, LIAO Haizhou, JI Wei, LI Qian   

  1. (College of Science, Gansu Agricultural University, Lanzhou 730070, China)
  • Online:2023-08-25 Published:2023-09-01

摘要: 以酪蛋白胶束和海藻酸钠为基质,以葡萄糖酸-δ-内酯(gluconate-δ-lactone,GDL)为酸化试剂,制备含油70%的O/W型乳液凝胶,研究GDL添加量对乳液凝胶微观结构、稳定性及流变性的影响,分析乳液凝胶对原花青素(proanthocyanidins,PC)的释放特性。结果表明,随着GDL添加量的增加,乳液凝胶中油滴的平均粒径减小,且油滴粒径在贮藏期内逐渐降低。GDL酸化改善了乳液凝胶的表观黏度和储能模量,二者随GDL添加量的增加而增大。乳液凝胶经65、80 ℃热处理30 min后结构未发生显著改变,且具有较高的酸碱稳定性和贮藏稳定性。另外,经乳液凝胶负载后,PC在模拟肠液消化过程中的释放率高于游离PC,其10 h累计释放率随GDL添加量的增加而增大。研究结果可为酪蛋白胶束基乳液凝胶制备及PC稳定性提升提供参考依据。

关键词: 酪蛋白胶束;原花青素;乳液凝胶;流变性;稳定性

Abstract: In this work, oil-in-water (O/W) emulsion gels with 70% oil phase were prepared using micellar casein (MC) and sodium alginate (SA) as substrates with the addition of gluconate-δ-lactone (GDL) as acidifying agent. The effects of GDL addition on the microstructure, stability and rheological properties of emulsion gels were studied, and the release characteristics of emulsion gels loaded with proanthocyanidins (PC) were analyzed. The results showed that with increasing GDL concentration, the average size of oil droplets in emulsion gels decreased, and it gradually decreased during the storage period. GDL acidification improved the apparent viscosity and storage modulus of emulsion gels, and this effect increased with GDL concentration. The microstructure of emulsion gels did not change significantly after heat treatment at 65 or 80 ℃ for 30 min. Meanwhile, the emulsion gels were stable to acidic and basic conditions as well as storage. In addition, the release rate of PC-loaded emulsion gels was higher than that of free PC during simulated intestinal digestion, and the cumulative release rate at 10 h increased with GDL concentration. The results of this study could provide a reference for the preparation of emulsion gels based on MC and the improvement of PC stability.

Key words: micellar casein; proanthocyanidins; emulsion gel; rheology; stability

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