食品科学 ›› 2024, Vol. 45 ›› Issue (1): 109-117.doi: 10.7506/spkx1002-6630-20230324-238

• 成分分析 • 上一篇    

毛健绿茶化学成分及促消化功能分析

高健健,周正阳,宋燕华,于帅,鹿伟,林智,戴伟东   

  1. (1.农业农村部茶树生物学与资源利用重点实验室,中国农业科学院茶叶研究所,浙江 杭州 310008;2.山西中医药大学第三临床学院,山西 晋中 030619;3.浙江省疾病预防控制中心,浙江 杭州 310008)
  • 发布日期:2024-02-05
  • 基金资助:
    浙江省自然科学基金杰出青年项目(LR23C160002)

Chemical Constituents and Digestion-Promoting Effect of Maojian Green Tea

GAO Jianjian, ZHOU Zhengyang, SONG Yanhua, YU Shuai, LU Wei, LIN Zhi, DAI Weidong   

  1. (1. Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese of Agricultural Academy Sciences, Hangzhou 310008, China; 2. Third Clinical College, Shanxi University of Chinese Medicine, Jinzhong 030619, China; 3. Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310008, China)
  • Published:2024-02-05

摘要: 采用保健食品功效学研究中的小鼠小肠运动评价,大鼠体质量、体质量增量、摄食量、食物利用率评价、大鼠胃蛋白酶活性及胃蛋白酶排出量测定等实验评估毛健绿茶水提物的促消化效果,并进一步结合基于超高效液相色谱-四极杆-静电轨道阱质谱的代谢组学方法对其化学成分进行系统解析。动物实验结果表明,与模型对照组相比,高剂量毛健绿茶水提物组(0.83 g/(kg·d))小鼠小肠墨汁推进率显著升高(P<0.05),与阴性对照组(去离子水组)相比,中剂量毛健绿茶水提物组(0.21 g/(kg·d))大鼠胃蛋白酶排出量显著升高(P<0.05),结合两者结果表明毛健绿茶具有一定的促消化功能;代谢组学分析在毛健绿茶水提物中共鉴定出98 个化合物,其中黄酮(芹菜素和木犀草素,0.14~0.77 mg/g)、黄烷酮(柚皮素和圣草酚,0.49~1.49 mg/g)、黄酮-7-O-葡萄糖苷(0.57~9.07 mg/g)和黄烷酮-7-O-葡萄糖苷(4.49~38.98 mg/g)为主要成分。本研究较为系统地评价了毛健绿茶水提物的促消化功能,并解析其主要化学成分,为日后毛健绿茶及其新产品的推广和开发利用提供了相应的理论依据。

关键词: 毛健绿茶;化学成分;代谢组学;液相色谱-质谱联用;促消化功能

Abstract: In this study, the digestion-promoting function of an aqueous extract from Maojian green tea extract (MJ-GTE) was evaluated by small intestinal motility in mice as well as body mass, body mass gain, food intake, food utilization rate, gastric pepsin activity, and gastric pepsin excretion in rats. The chemical composition of MJ-GTE was then systematically analyzed using metabolomics based on ultra-high performance liquid chromatography-quadrupole electrostatic orbitrap mass spectrometry (UPLC-Q-Exactive/MS). The results of animal experiments showed that the intestinal propulsion ratio of ink in the high-dose MJ-GTE group (0.83 g/(kg·d)) was significantly increased compared with the model group (P < 0.05), and gastric pepsin excretion in the medium-dose MJ-GTE group (0.21 g/(kg·d)) was significantly increased compared with the negative control group (deionized water) (P < 0.05), which collectively indicated that MJ-GTE has a digestion-promoting effect. The metabolomics analysis identified 98 compounds, among which, flavones (apigenin and luteolin, 0.14–0.77 mg/g), flavanones (naringenin and eriodictyol, 0.49–1.49 mg/g), flavone-7-O-glycosides (0.57–9.07 mg/g), and flavanone-7-O-glycosides (4.49–38.98 mg/g) were the major components in MJ-GTE. This study will provide a theoretical basis for the promotion and development of Maojian green tea and related products in the future.

Key words: Maojian green tea; chemical composition; metabolomics; liquid chromatography-mass spectrometry; digestion-promoting effect

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