食品科学 ›› 2024, Vol. 45 ›› Issue (2): 268-273.doi: 10.7506/spkx1002-6630-20230330-309

• 成分分析 • 上一篇    下一篇

秭归丝绵茶鲜叶非挥发性成分及丝形态结构分析

刘璇,李玉川,宋育林,雷震东,李艳,余志,倪德江,陈玉琼   

  1. (1.果蔬园艺作物种质创新与利用全国重点实验室,华中农业大学,湖北 武汉 430070;2.华中农业大学园艺林学学院,湖北 武汉 430070;3.秭归县农业农村局,湖北 宜昌 443600)
  • 出版日期:2024-01-25 发布日期:2024-02-05
  • 基金资助:
    学校地方合作项目-丝绵茶研究项目(707119028)

Non-volatile Components and Filamentary Morphology of Fresh Leaves of Zigui Simian Tea

LIU Xuan, LI Yuchuan, SONG Yulin, LEI Zhendong, LI Yan, YU Zhi, NI Dejiang, CHEN Yuqiong   

  1. (1. National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, China;2. College of Horticulture & Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China;3. Zigui County Agricultural and Rural Bureau, Yichang 443600, China)
  • Online:2024-01-25 Published:2024-02-05

摘要: 为研究丝绵茶品质形成特点,以秭归当地生产丝绵茶的茶树种‘丝绵土茶’6 个嫩度部位叶片为研究对象,通过超高效液相色谱飞行时间质谱、电感耦合等离子体发射光谱和扫描电子显微镜,分析其非挥发性代谢成分、矿质元素、“丝”结构及数量特点。结果表明,丝绵茶鲜叶中的非挥发性代谢成分在嫩度较高的叶位富集较多,其中一叶和二叶(L1、L2)中氨基酸、生物碱、儿茶素和香气糖苷物质含量较高;茶氨酸、有机酸和黄酮类在第三叶(L3)富集最多。而嫩度较低的五六叶中非挥发性成分含量相对较低。不同嫩度鲜叶原料积累的各种元素具有明显差异,氮、磷、钾、锌和铜元素在嫩度较高的一、二叶位(L1、L2)含量较高,L1分别为32.41 mg/g、4.53 mg/g、15.65 mg/g、45.45 μg/g、10.75 μg/g,L2分别为30.60 mg/g、3.70 mg/g、14.12 mg/g、35.82 μg/g、9.02 μg/g;而铁、锰和钙在成熟叶位含量较高。通过扫描电镜观察发现丝绵茶鲜叶中“丝”结构包括三股卷曲和单股卷曲两种形式,分布在主脉和侧脉的维管束木质部内螺纹或环纹导管;且二、三、四叶中“丝”的数量较芽头和一叶多。

关键词: 丝绵茶;嫩度;非挥发性成分;矿质元素;“丝”结构

Abstract: To investigate the characteristics of quality formation of Simian tea, the non-volatile components, mineral elements and filamentary structure of fresh leaves from different positions of ‘Simian Tucha’ tea plants, endemic to Zigui, were analyzed using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), inductively coupled plasma optical emission spectrometry (ICP-OES), and scanning electron microscopy (SEM). The results showed that the non-volatile components were more abundant in the leaves with higher tenderness, with amino acids, alkaloids, catechins, and aroma glycosides being mainly concentrated in the first (L1) and second leaves (L2). Theanine, organic acids, and flavonoids were mainly concentrated in the third leaf (L3), while the content of non-volatile components in the older leaves was relatively lower. The accumulation of mineral elements greatly varied among tea leaves with different tenderness. The contents of nitrogen, phosphorus, potassium, zinc, and copper in L1 (32.41 mg/g, 4.53 mg/g, 15.65 mg/g, 45.45 μg/g, and 10.75 μg/g, respectively) and L2 (30.60 mg/g, 3.70 mg/g, 14.12 mg/g, 35.82 μg/g, 9.02 μg/g, respectively) were higher than those in older leaves. The contents of iron, manganese, and calcium were higher in mature leaves. Through SEM observation, it was found that the filamentary structure of Simian tea leaves consisted of two forms, triple-stranded coil and single-stranded coil, and was distributed in the internal thread or ringed vessels of the xylem in the vascular bundles in the midvein and lateral veins, and the number of filamentary structures was higher in the second, third and fourth leaves than that in buds and L1.

Key words: Simian tea; tenderness; non-volatile components; mineral elements; filamentary structure

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