食品科学 ›› 2024, Vol. 45 ›› Issue (13): 146-152.doi: 10.7506/spkx1002-6630-20231020-156

• 营养卫生 • 上一篇    

4 株双歧杆菌对秀丽隐杆线虫的体内抗衰老作用

龚春淼, 王晓楠, 费鹏, 李岩, 赵小萌, 段泊安, 陈树兴,   

  1. (1.河南科技大学食品与生物工程学院,河南 洛阳 471023;2.中原食品实验室,河南 漯河 462333;3.南阳理工学院张仲景国医国药学院,河南 南阳 473000;4.漯河食品职业学院,河南 漯河 462333)
  • 发布日期:2024-07-12
  • 基金资助:
    北京市科技计划项目(Z201100008020005)

Anti-aging Effects of Four Bifidobacterium Species on Caenorhabditis elegans

GONG Chunmiao, WANG Xiaonan, FEI Peng, LI Yan, ZHAO Xiaomeng, DUAN Bo’an, CHEN Shuxing   

  1. (1. College of Food & Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; 2. Food Laboratory of Zhongyuan, Luohe 462333, China; 3. Zhang Zhongjing School of Chinese Medicine, Nanyang Institute of Technology, Nanyang 473000, China; 4. Luohe Vocational College of Food, Luohe 462333, China)
  • Published:2024-07-12

摘要: 为探究4 株双歧杆菌(假小链双歧杆菌BP-1、长双歧杆菌BL-3、动物双歧杆菌BA-5、婴儿双歧杆菌BI-38)对秀丽隐杆线虫的体内抗衰老作用,测定4 株双歧杆菌对线虫寿命、生殖能力、活力、体形、抗逆性、体内脂褐素水平、抗氧化酶活力和丙二醛含量的影响。结果表明,与空白对照组(大肠杆菌OP50)相比,BA-5、BI-38有效地延长了线虫的寿命(P<0.05),BP-1、BL-3对线虫寿命影响不显著(P>0.05);同时BA-5、BI-38并不会减少线虫的产卵量和削弱线虫的运动能力(P>0.05),且能有效增强其吞咽能力并显著减小体形(P<0.05);BA-5、BI-38能够有效增强线虫的抗热应激和抗氧化应激能力进而延长其寿命(P<0.05),显著减少体内脂褐素积累和降低丙二醛含量(P<0.05),并显著提高线虫体内的2 种抗氧化酶(过氧化氢酶、超氧化物歧化酶)的活力(P<0.05)。综上,婴儿双歧杆菌BI-38和动物双歧杆菌BA-5具有更强的体内抗衰老活性。

关键词: 双歧杆菌;秀丽隐杆线虫;抗衰老;体内

Abstract: The anti-aging effects of four Bifidobacterium species (B. pseudospermum BP-1, B. longum BL-3, B. animalis BA-5 and B. infantis BI-38) on Caenorhabditis elegans were evaluated in terms of its lifespan, reproductive capacity, vitality, body shape, stress resistance, lipofuscin levels, antioxidant enzyme activity and malondialdehyde (MDA) content. The results showed that compared with the control group (Escherichia coli OP50), BA-5 and BI-38 effectively extended the lifespan of C. elegans (P < 0.05), while BP-1 and BL-3 had no significant effect on the lifespan of C. elegans (P > 0.05). Meanwhile, BA-5 and BI-38 did not impair the reproductive or locomotory capacities of C. elegans (P > 0.05), but could effectively enhance its pharyngeal pumping and reduce its body size (P < 0.05). BA-5 and BI-38 were able to enhance tolerance to heat or oxidative stress and thus prolong the lifespan of C. elegans (P < 0.05), significantly reduce lipofuscin accumulation and MDA content (P < 0.05), and significantly increase the activity of two antioxidant enzymes (catalase and superoxide dismutase) in C. elegans (P < 0.05). In conclusion, B. infantis BI-38 and B. animalis BA-5 had stronger anti-aging activity in vivo.

Key words: Bifidobacterium; Caenorhabditis elegans; anti-aging; in vivo

中图分类号: