关键词: 蓝莓；玻璃态；冷冻状态；品质；冻藏

Abstract: Frozen ‘Beicun’ blueberry fruits were classified based on three key temperatures: the glass transition temperature (Tg’) at their maximum cryo-concentration, the intersection temperature (Tg”) between the freezing curve and the glass transition temperature curve, and the characteristic freezing endpoint temperature (Tm’) into three states: rubbery (T > Tm’), partially frozen concentrated (Tg” < T < Tm’), and glassy (T < Tg”). Blueberry fruits were quickly frozen in liquid nitrogen at −120 ℃ and separated into two storage groups. One group, without any state changes, was stored under rubbery (−18 ℃, T > Tm’), partially frozen concentrated (−40 ℃, Tg”< T < Tm’), or glassy state (−80 ℃, T < Tg”) conditions. The other group, with state changes, was initially stored at the glassy state temperature (−80 ℃) and then switched to the rubbery (−30 ℃, T > Tm’), partially frozen concentrated (−40 ℃, Tg” < T < Tm’), or lower glassy state temperature (−50 ℃, Tg’ < T < Tg”; −60 ℃, T < Tg’). Besides, a control group was set up without any temperature changes. Juice loss, hardness, nutrient contents, membrane integrity, and enzyme activities were determined to explore the effects of different freezing states and state changes on the quality of frozen blueberry fruits. The results indicated that frozen blueberry fruits in the glassy state exhibited the best quality. State transitions resulted in more pronounced quality deterioration in blueberry fruits; greater temperature changes led to higher juice loss, hardness reduction and nutrient loss, more serious cell integrity damage, and higher activities of peroxidase and polyphenol oxidase. Therefore, glassy state freezing (at temperatures below Tg’) could better maintain the quality of blueberry fruits; however, it is crucial to avoid state transitions caused by temperature fluctuations during frozen storage.

Key words: blueberry; glassy state; freezing states; quality; frozen storage