河鲀鱼皮源促胆囊收缩素分泌肽的制备及结构鉴定

doi:10.7506/spkx1002-6630-20241111-075

食品科学 ›› 2025, Vol. 46 ›› Issue (12): 156-162.doi: 10.7506/spkx1002-6630-20241111-075

河鲀鱼皮源促胆囊收缩素分泌肽的制备及结构鉴定

宋洪东，任韶霞，邵诸伟，葛少成，乔琨，刘智禹

（1.上海理工大学健康科学与工程学院，上海 200093；2.福建水产研究所，国家海水鱼类加工技术研发分中心（厦门），福建省海洋生物增养殖与高值化利用重点实验室，福建?厦门 361013）

出版日期:2025-06-25 发布日期:2025-05-23
基金资助:
福建省海洋生物增养殖与高值化利用重点实验室开放课题资助项目（2021fjscq12）；上海市自然科学基金面上项目（23ZR1444500）

Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish

SONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu

(1. School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; 2. National R&D Center for Marine Fish Processing (Xiamen), Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, Xiamen 361013, China)

Online:2025-06-25 Published:2025-05-23

摘要/Abstract

摘要： 为获得具有刺激胆囊收缩素（cholecystokinin，CCK）分泌活性的胶原肽，本研究以河鲀鱼皮为原料提取胶原蛋白，利用碱性蛋白酶对其进行水解以获得胶原肽，并采用体外肠内分泌STC-1细胞模型评价不同水解度胶原肽的促CCK分泌活性。结果显示，当水解度为12%时，所制备的胶原肽具有最高的促CCK分泌活性。进一步采用YMC ODS C18色谱柱对胶原肽进行分离纯化，并利用液相色谱串联质谱技术鉴定肽序列，成功鉴定出6 条肽序列。其中，LSGK、LPGKPGL和KMTPT 3 条肽具有显著的促CCK分泌活性。本研究可为河鲀鱼皮肽在调控肠道CCK分泌方面的应用提供理论基础，为河鲀鱼皮肽功能产品开发提供新思路。

关键词: 河鲀鱼皮；胶原肽；胆囊收缩素；STC-1细胞；分离纯化；结构鉴定?

Abstract: To obtain collagen peptides with the ability to stimulate cholecystokinin (CCK) secretion, collagen was extracted from puffer fish skin and hydrolyzed with alkaline protease. The CCK secretion-promoting activity of collagen peptides with different hydrolysis degrees was evaluated using an in vitro enteroendocrine STC-1 cell model. The results showed that the peptide with a hydrolysis degree of 12% had the highest CCK secretion-promoting activity. This peptide was separated and purified using a YMC ODS C18 column. Six peptide sequences were identified using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among them, three peptides, LSGK, LPGKPGL and KMTPT, had significant CCK secretion-promoting activity. This study provides a theoretical basis for the application of puffer fish skin peptides in regulating intestinal CCK secretion and provides new ideas for the development of functional products based on puffer fish skin peptides.

Key words: puffer fish skin; collagen peptide; cholecystokinin; STC-1 cells; separation and purification; structural identification

中图分类号:

S985.1＋3

宋洪东，任韶霞，邵诸伟，葛少成，乔琨，刘智禹. 河鲀鱼皮源促胆囊收缩素分泌肽的制备及结构鉴定[J]. 食品科学, 2025, 46(12): 156-162.

SONG Hongdong, REN Shaoxia, SHAO Zhuwei, GE Shaocheng, QIAO Kun, LIU Zhiyu. Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish[J]. FOOD SCIENCE, 2025, 46(12): 156-162.

河鲀鱼皮源促胆囊收缩素分泌肽的制备及结构鉴定

Preparation and Structural Identification of Peptides Stimulating Cholecystokinin Secretion from the Skin of Puffer Fish

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