酒糟源黄嘌呤氧化酶抑制肽的虚拟筛选及其对高尿酸小鼠的降尿酸作用

doi:10.7506/spkx1002-6630-20250620-152

食品科学 ›› 2025, Vol. 46 ›› Issue (24): 200-207.doi: 10.7506/spkx1002-6630-20250620-152

• 营养卫生 • 上一篇

酒糟源黄嘌呤氧化酶抑制肽的虚拟筛选及其对高尿酸小鼠的降尿酸作用

林晓婕，王瑛，梁立杰，王培鑫，严锦贤，梁璋成，何志刚，李维新

（1.福建省农业科学院农产品加工研究所，福建省农产品（食品）加工重点实验室，农业农村部亚热带特色果蔬菌加工重点实验室（部省共建），福建福州 350003；2.福建省农业科学院农业质量标准与检测技术研究所，福建福州 350003；3.福建中医药大学附属第二人民医院，福建省医疗机构中药制剂重点实验室，福建福州 350003）

发布日期:2025-12-26
基金资助:
福建省公益类竞争性项目（2024R1081）；福建省公益类自选项目（2024R1031002）

Xanthine Oxidase Inhibitory Peptide from Distiller’s Grains: Virtual Screening and Uric Acid-Lowering Effect

LIN Xiaojie, WANG Ying, LIANG Lijie, WANG Peixin, YAN Jinxian, LIANG Zhangcheng, HE Zhigang, LI Weixin

(1. Fujian Key Laboratory of Agricultural Product (Food) Processing, Key Laboratory of Subtropical Characteristic Fruits, Vegetables and Edible Fungi Processing (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 2. Institute of Quality Standards & Testing Technology for Agro-products, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 3. The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medicine in Medical Institutions of Fujian Province, Fuzhou 350003, China)

Published:2025-12-26

摘要/Abstract

摘要： 目的：为实现酒糟的高附加值利用，挖掘酒糟源黄嘌呤氧化酶（xanthine oxidase，XOD）抑制肽，明确肽段对高尿酸血症（hyperuricemia，HUA）小鼠的降尿酸功效。方法：以白酒酒糟酶解物为研究对象，采用超滤分离、液相色谱-质谱联用技术和计算机分析筛选潜在的XOD抑制肽，通过分子对接阐释作用机理，并评价对HUA小鼠的降尿酸作用。结果：＜1 kDa组分的XOD抑制活性最佳；经鉴定和筛选得到4 条XOD抑制肽，分别为WDLPF、WPQ、WFPE和LQKW，其中活性最佳的肽段为LQKW，其对XOD活性的半数抑制浓度为2.70 mg/mL，经胃肠消化后XOD抑制活性保持率为（61.84±0.82）%；LQKW与受体蛋白1FIQ主要通过氢键和疏水相互作用力结合；以LQKW干预HUA小鼠，与模型组相比，低、中、高剂量（200、400、800 mg/kg）LQKW组的尿酸水平分别极显著降低了32.59%、35.96%和37.28%（P＜0.01）；同时，中、高剂量LQKW组小鼠肾脏指数、肌酐和尿素氮水平极显著降低（P＜0.01），明显改善了HUA小鼠肾组织的病理损伤。结论：酒糟源XOD抑制肽LQKW通过氢键和疏水相互作用与1FIQ结合产生抑制活性，可有效降低HUA小鼠血清尿酸水平并发挥肾脏保护作用。本研究可为酒糟源降尿酸活性肽开发提供理论支撑。

关键词: 酒糟；黄嘌呤氧化酶抑制肽；虚拟筛选；分子对接；降尿酸?

Abstract: Objective: To achieve high-value utilization of distiller’s grains, this study explored xanthine oxidase (XOD) inhibitory peptides from distiller’s grains and determined their anti-hyperuricemic effect in mice. Methods: Potential XOD inhibitory peptides from an enzymatic hydrolysate of distiller’s grains were isolated by ultrafiltration and identified by liquid chromatography-mass spectrometry (LC-MS) and in silico analysis. The action mechanism of XOD inhibitory peptides was elucidated using molecular docking, and the anti-hyperuricemic effect in mice was evaluated. Results: The < 1 kDa fraction exhibited the highest XOD inhibitory activity. Four XOD inhibitory peptides were obtained, namely WDLPF, WPQ, WFPE, and LQKW. Among them, LQKW demonstrated the highest activity, with a half maximal inhibitory concentration (IC50) of 2.70 mg/mL. After gastrointestinal digestion, (61.84 ± 0.82)% of its activity remained. Molecular docking revealed that LQKW primarily bound to the receptor protein 1FIQ through hydrogen bonds and hydrophobic interactions. Compared with the model group, low-, medium-, and high-dose (200, 400, and 800 mg/kg) LQKW significantly (P < 0.01) reduced serum uric acid levels by 32.59%, 35.96%, and 37.28%, respectively. Additionally, medium- and high-dose LQKW significantly (P < 0.01) decreased kidney index, creatinine, and blood urea nitrogen levels while markedly alleviating renal pathological damage in hyperuricemic mice. Conclusion: The distiller’s grain-derived XOD inhibitory peptide LQKW exerts its inhibitory activity by binding to 1FIQ via hydrogen bonds and hydrophobic interactions, thereby effectively reducing serum uric acid levels in HUA mice and providing renal protection. This study provides theoretical support for the development of anti-hyperuricemic peptides from distiller’s grains.

Key words: distiller’s grains; xanthine oxidase inhibitory peptide; virtual screening; molecular docking; uric acid lowering

中图分类号:

林晓婕，王瑛，梁立杰，王培鑫，严锦贤，梁璋成，何志刚，李维新. 酒糟源黄嘌呤氧化酶抑制肽的虚拟筛选及其对高尿酸小鼠的降尿酸作用[J]. 食品科学, 2025, 46(24): 200-207.

LIN Xiaojie, WANG Ying, LIANG Lijie, WANG Peixin, YAN Jinxian, LIANG Zhangcheng, HE Zhigang, LI Weixin. Xanthine Oxidase Inhibitory Peptide from Distiller’s Grains: Virtual Screening and Uric Acid-Lowering Effect[J]. FOOD SCIENCE, 2025, 46(24): 200-207.

酒糟源黄嘌呤氧化酶抑制肽的虚拟筛选及其对高尿酸小鼠的降尿酸作用

Xanthine Oxidase Inhibitory Peptide from Distiller’s Grains: Virtual Screening and Uric Acid-Lowering Effect

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