食品科学 ›› 2002, Vol. 23 ›› Issue (10): 45-50.

• 基础研究 • 上一篇    下一篇

卷枝毛霉3.2208条件优化产类可可脂的研究

 董欣荣, 曹健, 雷银平, 赵斌   

  1. 郑州工程学院生物工程系
  • 出版日期:2002-10-15 发布日期:2011-12-31

Study on Covoa Butter Equivalent Production by Mucor Circinelloides 3.2208 in Optimum Condition

 DONG  Xin-Rong, CAO  Jian, LEI  Yin-Ping, ZHAO  Bin   

  • Online:2002-10-15 Published:2011-12-31

摘要: 通过正交实验,得出卷枝毛霉3.2208产类可可脂的最佳培养条件为:接种量0.5ml;温度33℃;pH值,6.0;C/N比,100;培养时间6d;氮源为蛋白胨;碳源为麦芽糖;FeSO4•7H2O0.01g/L;ZnSO4•7H2O0.01g/L;MgSO4•7H2O0.0005g/L;MgSO4•7H2O0.8g/L;油酸0g/L。在该条件下,卷枝毛霉的CBE得率为3.61%,油脂系数为0.29,其二位不饱和脂肪酸为79.08%。

关键词: 卷枝毛霉3.2208, 类可可脂, 优化培养条件

Abstract: Through the orthogonality experiment, the optimization culture condition for accumulating CBE by Mucor circinelloides 3.2208 was achieved as following:inoculum quantity 0.5ml;temperature 33℃;pH6.0;C/N ratio100;culture time 6 days;nitrogen source is peptone;carbon source is maltose;FeSO4•7H2O 0.01g/L;ZnSO4•7H2O 0.01g/L;MnSO4•4H2O 0.0005g/L;MgSO4•7H2O 0.8/L;Oleic 0g/L.Under such culture condition,we gain the Mucor circinelloides 3.2208 CBE 3.61%from dry cell.The lipid coefficient is 0.29,the Sn-2unsaturated fatty acid is up to 78.08%.

Key words: Mucor circinelloides 3.2208 , Cocoa Butter , Equivelent Optimal culture condition