食品科学 ›› 2002, Vol. 23 ›› Issue (10): 76-78.

• 工艺技术 • 上一篇    下一篇

酶法提取烤鳗下脚料中水解动物蛋白的研究

 黄志勇, 赖海涛, 许伟雄, 涂开生   

  1. 集美大学生物工程学院
  • 出版日期:2002-10-15 发布日期:2011-12-31

Investigation of Protease on Extraction of Hydrolyzed Animal Protein(HAP)from the Eel Scraps

 HUANG  Zhi-Yong, LAI  Hai-Tao, XU  Wei-Xiong, TU  Kai-Sheng   

  • Online:2002-10-15 Published:2011-12-31

摘要: 酶解提取烤鳗下脚料的水解动物蛋白是可行的,实验发现:在枯草杆菌蛋白酶、木瓜蛋白酶和胃蛋白酶这三种水解中,枯草杆菌蛋白酶的水解效率最高。通过正交试验,确定用枯草杆菌蛋白酶在50℃、pH7.5、固液比5∶8,添加酶量为下脚料中蛋白质含量的2.0%,反应时间为5h时,其水解度达82.6%。

关键词: 烤鳗下脚料, 水解动物蛋白(HAP), 水解度

Abstract: Introduced in this paper was the method of extraction of hydrolyzed animal protein (HAP) from eel scraps by protease. Through the ofthogonal tests of hydrolization with the three enzymes,namely papain, pepsinum and hay bacillus protease, it was found that the hay bacillus protease was the most effective protease for hydrolyzing HAP. The degree of hydrolysis (DH) by the hay bacillus protease was 82.6% under the optimum hydrolyzing conditions: 50℃, pH 5.0, ratio of scraps and water being 5:4, the protease content being 2.0% of the protein contained in the scraps, and the reaction time as 5 h.

Key words: Eel scraps , Hydrolyzed Animal Protein(HAP) , Degree of hydrolysis