磺基醋酸汞试剂法用于C21甾苷定量检测的可行性研究

食品科学 ›› 2002, Vol. 23 ›› Issue (10): 94-96.

磺基醋酸汞试剂法用于C21甾苷定量检测的可行性研究

宋俊梅, 刘红蕾, 丁霄霖

山东轻工业学院食工系，山东大学生命科学学院，江南大学食品学院

出版日期:2002-10-15 发布日期:2011-12-31

Fessibility Study on Method of C21 Steroidal Glycosides’ Quantitative Determination with Sulfonic Mercuric Acetate Reagent

SONG Jun-Mei, LIU Hong-Lei, DING Xiao-Lin

Online:2002-10-15 Published:2011-12-31

摘要/Abstract

摘要： 经实验确定了甾苷定量测定的官能团反应方法。用定量测定3-羟基族化合物的最特效的方法磺基醋酸汞试剂法直接测定C21甾苷元的C3-OH,从而间接测定C21甾苷,实验证明该方法是可行的。

关键词: C21甾苷, 3-羟基族化合物, 磺基醋酸汞试剂法

Abstract: In order to establish the quantitative determination method for C21 steroidal glycoside in Asclepiadaceae plants as homologous health food ,the functional group reaction method of C21 steroidal glycoside’s quantitative determination was studied in this paper after the research on their extraction method.The sulfonic mercuric acetate reagent method,which was unique to 3-hydrooxyl steroids,was used to determine the C3-OH on the aglycones directly in order to deternine the glycosides indirectly .The results showed that the method was feasible .

Key words: C21 steroidal glycosides, 3-hydrooxyl steroids , Sulfonic mercuric reagent method

宋俊梅, 刘红蕾, 丁霄霖. 磺基醋酸汞试剂法用于C21甾苷定量检测的可行性研究[J]. 食品科学, 2002, 23(10): 94-96.

SONG Jun-Mei, LIU Hong-Lei, DING Xiao-Lin. Fessibility Study on Method of C21 Steroidal Glycosides’ Quantitative Determination with Sulfonic Mercuric Acetate Reagent[J]. FOOD SCIENCE, 2002, 23(10): 94-96.

[1]	吕志敏1，田鹭1,2,*，朱卫华2. 超临界CO2萃取白首乌中的C21甾苷[J]. 食品科学, 2009, 30(12): 127-130.
[2]	宋俊梅, 丁霄霖. C21甾苷提取方法的研究[J]. 食品科学, 2002, 23(9): 61-65.