食品科学 ›› 2003, Vol. 24 ›› Issue (11): 81-83.

• 工艺技术 • 上一篇    下一篇

化学诱变选育低双乙酰啤酒酵母菌株的研究

 唐晓达, 张秀丽, 魏文铃, 刘月英, 刘进文   

  1. 厦门大学生命科学学院
  • 出版日期:2003-11-15 发布日期:2011-12-13

Study on Chemical Mutation of Selected Strain of Saccharomyces Carlsbergensis FB

 TANG  Xiao-Da, ZHANG  Xiu-Li, WEI  Wen-Ling, LIU  Yue-Ying, LIU  Jin-Wen   

  1. College of Life Science, Xiamen University
  • Online:2003-11-15 Published:2011-12-13

摘要: 通过EMS诱变,从啤酒酿造生产菌株啤酒酵母(Saccharomyces carlsbergensis)FB中筛选分离得到一株发酵液中双乙酰含量优于亲株的新菌株FB-E1。以120Bx麦芽汁为培养基,用内装300ml麦芽汁的500ml三角瓶于12℃下发酵,发酵8d后发酵液中双乙酰含量比亲株降低了42.7%。该菌株的其它发酵性能的测定结果表明其保持了亲株的优良性状,且遗传性状稳定。

关键词: 啤酒酵母, 诱变, 双乙酰

Abstract: After mutation with EMS (ethyl methane sulfonate), a Saccharomyces carlsbergensis strain FB-E1 was selected fromoriginal strain FB, a strain for beer brewage production. The diacetyl content in the fermented liquid during the fermentation wasinvestigated in 500ml flask with 300ml 120Bx wort at 11℃. After 8d, the diacetyl content in the fermented liquid of FB-E1 was0.0706mg/L, 42.7% lower than that of its original strain A (0.1233mg/L). The results showed that flocculence and fermentationrate of strain FB-E1 were kept just as good as the original strain FB.

Key words: brewer’s yeast, mutation, diacetyl