关键词: 五味子, 可溶性多糖, 提取工艺

Abstract: The paper studied the extraction of polysaccharide in Schisandra. Single factor test and orthogonal experiment designmethods L9(33) were applied to analyze the influence of the factors such as solid-liquid ratio and temperature and time on theextraction percent of the polysaccharides. Experimental results indicated that temperature and solid-liquid ratio would signifi-cantly affect the extraction percent of the soluble polysaccharides.The optimum solid-liquid ratio was: 1:25, temperature is 100℃ and the extraction time 4h.In this condition, the extraction percent of soluble polysaccharides in Schisandra was 5.38%. Inaddition, the traditional method of polysaccharides precipitated with alcohol was improved. And Sevag method combined withenzyme method based on traditional Sevag method to eliminate protein was adopted. This method greatly decreased the time ofeliminating protein. Also polysaccharide was assayed by improval anthroic ketone-sulphuric acid method.

Key words: Schisandra, soluble polysaccharide, extraction technology