食品科学 ›› 2004, Vol. 25 ›› Issue (6): 52-56.
• 基础研究 • 上一篇 下一篇
孙万成, 宋龄英, 蒋笃孝, 罗毅皓
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SUN Wan-Cheng, SONG Ling-Ying, JIANG Du-Xiao, LUO Yi-Hao
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摘要: 在固定化磷脂酶A1时,利用酶抑制剂与磷脂酶A1活性中心氨基酸残基结合,使戊二醛基与磷脂酶A1的非活性氨基残基缩合,从而起到保护酶活性中心基团,提高固定化酶活力作用。实验证明磷酸盐是磷脂酶A1的竞争性抑制剂,并求出Ki值。
关键词: 酶抑制剂, 固定化磷脂酶A1
Abstract: When immobilized PLA1, inhibitor can couple amine residues which are important for catalysis or maintaining proteinstructure. Therefore, it can protect amine groups of activating site by binding between glutaradehyde and inactive groups of amineso as to increase activity retention of immobilized enzyme.In addtion, phosphate is shown to be a competive inhibitor of PLA1,and by means of making chart to get the Ki value.
Key words: inhibitor, immobilized PLA1(phospholipase A1)
孙万成, 宋龄英, 蒋笃孝, 罗毅皓. 利用酶抑制剂固定化磷脂酶A1(PLA1)的研究[J]. 食品科学, 2004, 25(6): 52-56.
SUN Wan-Cheng, SONG Ling-Ying, JIANG Du-Xiao, LUO Yi-Hao. Inhibitor Study on Immobilized PLA1[J]. FOOD SCIENCE, 2004, 25(6): 52-56.
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